Why is Sanger sequencing sometimes referred to as "dye-terminator" sequencing?

Q: What is linker scanning mutagenesis?

A: Answer: Introduction: Old-style approaches, containing linker-scanning mutagenesis includes…

Q: Why is genome database and annotation relevant to Genome sequencing?   Are there any examples?

A: Genome: the set of genes. Genome database: it is the collection of all information on the human…

Q: What are the five main steps in DNA cut-and-paste transposition?

A: The "cut and paste" method is used for saving conversion. The enzyme transposase acts as a pair of…

Q: How are dideoxynucleotide triphosphates utilized in sequencing?

A: DNA is the genetic substance of all creatures. It is a double-helical structure made up of two…

Q: Why Sanger Sequencing Dependson DNA Polymerase?

A: DNA sequencing is used to determine the exact arrangement of the nucleotide bases adenine (A),…

Q: What is the principle of next generation sequencing?

A: The process of determining the nucleic acid sequence is known as DNA sequencing.

Q: What is Sanger sequencing used for?

A: DNA sequencing is defined as a process in which DNA’s nucleotide sequence is determined. The main…

Q: What are three benefits Ion torrent sequencing has over sanger sequencing

A: DNA is an important biomolecule in the cell as it is the genetic material for most of the organisms…

Q: In microbial genetics, what is referred to as Griffith effect?

A: Microbial genetics is a subject area within microbiology and genetic engineering. Microbial genetics…

Q: What is domain? And Why is it complicated to classify and study domains purely using sequence…

A: All the prokaryotic organisms belong to Archea or Bacteria while eukaryotic organisms belong to…

Q: Explain the difference between sequencing by chain termination and sequencing by synthesis. Which…

A: Chain termination process employs dideoxynucleoside triphosphates (ddNTPs) lacking OH (hydroxyl)…

Q: Why a multiple sequence alignment is needed for researchers? What inferences can be derived from…

A: When three or more biological sequences are aligned and studied together, it is referred to as…

Q: What is the purpose of shotgun sequencing?

A: Deoxyribonucleic acid (DNA) sequencing is the process of determining the nucleic acid sequence based…

Q: Why are the proteins cleaved into small fragments for protein sequencing?

A: Proteins are the end product of the molecular translation that codes the information present in the…

Q: Why is the technique used to clone mammals called somatic cellnuclear transfer?

A: A somatic cell is a cell that takes part in the composition of the body of an organism and divides…

Q: What does whole exome sequencing test for?

A: Exomes are part of the genome, which contains only the coding portions of the genes that is the…

Q: What does 'biological sequence database' refer to?

A: Bioinformatics aids in the storage and process of large biological information by the aid of…

Q: what are similarities and differences between chain-termination and reversible terminator…

A: Gene sequencing  aids in the development of gene therapy, a form of treatment that replaces faulty…

Q: Explain the sequencing-by synthesis (SBS) approach ?

A: One of the well-known states that are known as fundamental topics in Arithmetic is sequence and…

Q: What is the principle behind to Sanger sequencing?

A: Sanger sequencing is a method of DNA sequencing, which is based on selective incorporation of…

Q: What is the function of the chaotropic salt in DNA isolation?

A: These salts are strong protein denaturing agents.

Q: Please list all possible products in a sequencing reaction using ddGTP as a terminator based on the…

A: 1.As dd GTP is used, every time it encounters C base it will terminate. 2.When C is encountered with…

Q: Define about Sanger sequencing ?

A: Introduction:- The process of determining the sequence of nucleotide bases (As, Ts, Cs, and Gs) in a…

Q: What does massively parallel sequencing mean?

A:

Q: What is a read (Sanger sequencing)?

A: DNA sequencing is the process of determining the sequence of nucleotides (adenine, guanine,…

Q: How are different variable region sequences generated?

A: Ans: The variable regions of the antibody have two chains heavy and light chains. They form a part…

Q: Why is it necessary to use only the Klenow fragment, rather than intact E. coli Pol I, in DNA…

A: A protein fragment that is obtained when the DNA polymerase I of E.coli is enzymatically cleaved…

Q: What advantages do cDNA libraries provide over genomic DNA libraries? Describe cloning applications…

A: DNA is the genetic material in most living organisms. It is the information hub of the cell that…

Q: What is the purpose of the dideoxynucleotide triphosphates in the Sanger sequencing reaction?

A: In 1977, Sanger proposed chain termination method of DNA sequencing. He used ddNTP or…

Q: What is the principle of Sanger sequencing?

A: DNA sequencing is the process of determining the sequence of nucleotides (adenine, guanine,…

Q: What key structural features of the DNA molecule underlie its ability to be faithfully replicated?

A: DNA is known as deoxyribonucleic acid. DNA is the genetic material in humans and other organisms.…

Q: Why does reversible chain termination sequencing yield short reads?

A: In genetics, sequencing is considered a methodology to determine the order of nucleotides(adenine,…

Q: How is genomic DNA prepared for shotgun sequencing?

A: Genomic DNA prepared for shotgun sequencing as follows :  1. ligating random genomic DNA fragments…

Q: Describe the difference between Sanger based sequencing and Next Generation Sequencing (NGS). Why is…

A: Step 1 Sequencing is the establishment of the primary structure of an unbranched polymer. Sequencing…

Q: What is the difference between a map-based approach to sequencing a whole genome and a whole-genome…

A: Deoxyribonucleic acid (DNA) is a double stranded helical genetic material containing thousands of…

Q: What is the purpose of the dideoxyribonucleoside triphosphates in the dideoxy-sequencing reaction?

A: DNA sequencing is carried out using various methods. The majorly used method of DNA sequencing is…

Q: What are frame-shift insertion?

A: Mutations are changes that occurs in the deoxyribonucleic acid (DNA) sequence, either due to…

Q: When isolating DNA, why is it helpful to control environmental conditions?

A: The ability to extract DNA is of primary importance to study the genetic causes of disease and for…

Q: Hi, I would like to know which program is used for the graphical presentation of the results of a…

A: A genome wide linkage association study (GWAS) is an approach used in genetic research to associate…

Q: Why is RNA sequencing important?

A: RNA or ribonucleic acid is a polymer of ribonucleotides connected together via a phosphodiester…

Q: What is a Sanger Sequencing ?

A: The term DNA sequencing encompasses biochemical methods for determining the order of the nucleotide…

Q: Explain how Sanger sequencing works

A: Genome sequencing: genome means total gene content present in the organism and sequence means to…

Q: What is the primary disadvantage of Sanger sequencing?

A: Sanger sequencing the target DNA sequence is determined by copying it into the fragments of…

Q: Why is bioethics such an essential area in genomics?

A: Bioethics: it is nothing but the study of social , legal and philosophical issues arising from…

Q: What key molecules are essential for Sanger sequencing?

A: DNA sequencing is used to determine the exact arrangement of the nucleotide bases adenine (A),…

Q: What is the significance of digesting the 16S rRNA product in 16s gene sequencing?

A: The 16S rRNA is a structural component of the 30S ribosomal subunit of  bacteria and archea.

Q: If you have access to the necessary computer software, make asequence file and analyze it in the…

A: The sequencing of genetic information is important for proper study and analysis, and with the…