Make oleic acid vesicles by making a solution of 0.5mL of 50mM oleic acid, 1 mM HPTS in 0.2 M Tris-HCl, 50 mM NaCl, pH 8.5 (i.e. buffer A) in a microcentrifuge tube in the following order. How much HPTS should you add? How much oleic acid?
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