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Methyl red testDoes a Phenol red organism have to be methyl red?


Methyl red test

Does a Phenol red organism have to be methyl red?

Step 1

It is required to find if the Phenol Red organisms are also Methyl Red in the indicator tests.

Step 2

The methyl red test and the Voges-Proskauer (VP) broth is a part of the IMViC test as the medium is such that both the tests can be performed on them. The IMViC test consists of a series of four tests for each abbreviated letter I- Indole production, M- Methyl Red test, V- Voges-Proskauer test and C- Citrate production test. These tests are used for differentiating the Enterobacteriaceae species.

The Methyl Red test is carried out by addition of pH indicator methyl red to an inoculated tube of the Methyl Red- Voges-Proskauer broth. If there are acidic end products present in the inoculums and methyl red is added then the methyl red remains red.

Step 3

Methyl Red is different from Phenol Red. Phenol Red is used for fermentation plates and Mannitol salt agar plates as a pH indicator. Phenol red turns yellow at a pH of 6.8 and fuchsia at pH above 7.4. If the byproduct formed in media by the utisation of carbohydrates and is aci...

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