1 2 3 4 M 1000 b 900 bp 800 bp 700 bp 600 bp 500 bp 400 bp 300 bp 200 bp

Q: According to Chargaff's observations of nucleotide composition of DNA samples OA % of (G + C) + % of…

A: Chargaff's rule has been stated for the DNA, which states that the ratio of purine and pyrimidine…

Q: nucleoside is composed of a) a base+ a sugar b) a base+ a sugar+ phosphate c) a base+ a phosphate…

A: Nucleotides and nucleosides make up nucleic acids (DNA and RNA). Both nucleotides and nucleosides…

Q: In primer designing, which of the following statements is correct? a. Primers should be 18-24 bases…

A: Primer is a short synthetic oligonucleotide which is used in many molecular techniques from PCR to…

Q: Which set of DNA fragments below would require a polyacrylamide gel to effectively separate the…

A: Polyacrylamide gel electrophoresis is a technique used to separate proteins or nucleic acids on the…

Q: What allows the peaks to be different colors?

A: DNA sequencing refers to the determination of the nucleotide sequence in the DNA molecule. Automated…

Q: A piece of DNA is cut into four fragments as shown below. A solution containing the four fragments…

A: Electrophoresis is a technique used to separate charged particles like protein, RNA, and DNA. It is…

Q: How is a DNA viewed on an agarose gel?

A: DNA stands for deoxyribonucleic acid. On an agarose gel, DNA is stained with the ethidium bromide.…

Q: samples Marker B 1,200 bp 1,000 bp 900 bp 800 bp 700 bp 600 bp s00 bp 400 bp 300 bp 200 bp 100 bp…

A: The given diagram is a representation of the process of gel electrophoresis. It is a laboratory…

Q: When you are extracting DNA one of the first steps is to dissolve the cell membranes with ice cold…

A: Gene expression: The process in which genetic information encoded in genes is expressed in the form…

Q: I have a 1.270 ug/uL dna stock, how would you dilute this to 50ng/nL in a single dilution step

A: Formula - 1 ug = 1000 ng Dilution = initial volume / final volume = final concentration / initial…

Q: Which of the following gels is the preferred choice for separation of DNA fragments?  Agarose…

A: There are several techniques which are used in recombinant DNA technology. In a recombinant DNA…

Q: The technique that Rosalind Franklin used to take images of DNA is called NMR. O a. False O b. True

A: Nucleic acids are polynucleotides that contain many nucleotides that are bound by phosphodiester…

Q: DNA concentration is 3.75 ng/ ul = the dilution factor is 1:______ we add____ uL dna extract to ____…

A: DNA shows an absorbance maxima at 260 nm and the absorbance is proportional to the concentration of…

Q: If the purity of DNA sample is below 1.8 A260/A280, where did the protein contamination come from?

A: The absorbance ratio at 260 nm & 280 nm is used to determine the quality of DNA & RNA.  A…

Q: 3000 bp 2000 bp 1000 bp 800 bp 700 bp 500 bp L Uncut EcoRI BamHI Ncol EcoR1/BamH1 BamHI/Nco1…

A: Plasmids are double-stranded circular DNA molecules. Due to their small size and stability, these…

Q: What do you mean by polyacrylamide gel electrophoresis (PAGE)  technique ?

A: To explain: To explain about polyacrylamide gel electrophoresis (PAGE)  technique and its uses

Q: All of the following are examples of materials that are bound by your purifying medium during the…

A: DNA is the genetic material in most living organisms. It is the information hub of the cell that…

Q: What volume of water is needed to make the following primer stocks solutions? Primer 1. 25 nM…

A: DNA Replication is the process by which one double-stranded DNA molecules gets replicated to produce…

Q: Using paper cutouts of the nucleotides, Watson compared the ___ of the base pairs.   A. hydrogen…

A: Watson and Crick give the double-helical structure of DNA. Two ribbon-like structure depicts the…

Q: DNA concentration in cuvette solution (in microgram per ml) Dilution factor of DNA sample in cuvette…

A: DNA is the nucleic acid and it absorbs the UV light with maximum absorbance at 260 nm wavelength due…

Q: Four nucleic-acid samples are analyzed to determine the percentages of nucleotides they contain.…

A: DNA and RNA are nucleic acids present in the organisms. DNA is the deoxy ribose nucleic acid whereas…

Q: During gel electrophoresis, in what direction (toward which electrode) does DNA move toward?…

A: There are different molecular biology tools and techniques used to analyze and quantify…

Q: In your forensic laboratory, you set up a reaction to digest DNA with restriction enzymes. The final…

A: In molecular cloning techniques like PCR and restriction cloning, restriction enzyme digestion is…

Q: What is the base at offset 5 of GATTACA? Remember: the leftmost base has offset 0. Select one: O 1.…

A: Correct option is 3. G (C = G) C present at offset 5.

Q: A piece of DNA is cut into four fragments as shown below. A solution containing the four fragments…

A: Gel electrophoresis is a technique used to separate different pieces of DNA on the basis of their…

Q: Why does DNA appear when you add cold alcohol to the strawberry DNA solution?

A:

Q: With regard to Chargaff’s experiment described in Figure shown,answer the following:A. What is the…

A: Erwin Chargaff carried out an experiment to determine the base composition of DNA. He concluded that…

Q: Find the connection among the words below and choose the letter of the word which is different. A.…

A: Ge electrophoresis Gel electrophoresis is a molecular technique used to separate the DNA fragments…

Q: If the stop nucleotides were not added prior to loading the samples in the gel, what would the…

A: Gel electrophoresis is a molecular biology technique used to separate the fragments of nucleotide…

Q: The following image is of an agarose gel. If DNA samples were loaded to this gel and the…

A: Electrophoresis is a method that uses an electric field to separate macromolecules in a fluid or gel…

Q: What is the function of each reagent used in the extraction of DNA from banana?  •Saltwater…

A: The extraction of DNA from any cell involves the following steps: Homogenization of the cells to…

Q: A piece of DNA is cut into four fragments as shown below. A solution containing the four fragments…

A: Electrophoresis is a laboratory technique used to separate DNA, RNA, or protein molecules based on…

Q: DNA is visualized during agarose gel electrophoresis by ______________ . the fact that DNA…

A: Agarose gel electrophoresis is a process of separation of DNA on the basis of mass and charge of…

Q: Results obtained from a Nano Spec. DNA samples collected: ng/uL A260/A80 A260/A230 E.coli genomic…

A: Beer-Lambert Law is applied in absorption spectrometry for biochemical analysis of various…

Q: A description of the principles of agarose gel electrophoresis of DNA.

A: INTRODUCTION:- Gel electrophoresis separates DNA on the basis of its charge and size in a solid…

Q: What technique was used to photograph DNA in franklins famous photo #51? A- gamma ray photography B-…

A: Imaging with gamma rays is used in nuclear medicine, as well as in court medicine. This technique…

Q: Please briefly explain what gel electrophoresis is and how it works to separate a mixed sample of…

A: Gel electrophoresis is a technique which is used to separate the molecules under the application of…

Q: What are the importance of accurate wavelength measurement in purified DNA sample? Explain why…

A: DNA can be extracted by using different protocols. Protocols of DNA isolation are modified according…

Q: Identify the level of DNA structure associated with the sequence of nucleotides. tertiary secondary…

A: Answer:- The correct answer for this question is Primary structure. The nucleotides which when…

Q: For a DNA sample, the OD value at 260nm = 0.125, and the OD value at 280nm 0.065. %3D Purity of DNA…

A: Purity of a DNA sample is defined as the ratio of absorbance at 260 nm and 280 nm. If the ratio…

Q: The Evidence shows which suspect was at the crime scene? ( image of murder investigation) A)…

A: DNA bands can appear thick and thin in the agarose gel electrophoresis, thicker and darker DNA bands…

Q: Double stranded DNA is denatured in the presence of high heat low heat high pressure acidic…

A: According to Bartleby guidelines , we are required to attempt first question in case of multiple…

Q: The first base shown in this chromatogram is closest to to : : : : 71 MW Select an answer and…

A: Asked : Correct option for 1st base in the chromatogram is closest to which end of DNA.

Q: DNA EXTRACTION Materials: knife                                                               1…

A: DNA extraction: It is the method used for the plants and animals both. It is the most recent and…

Q: 1. Electrophoresis is a method of (highlight the correct answer) (1) separating DNA fragments (2)…

A: The need to study different aspects of an organism’s physiological activities, cause of disease and…

Q: A 12 kb linear DNA fragment is subject to single or double RE digest and agarose electrophoresis, to…

A: Electrophoresis is a technique that causes charged particles to migrate and separate under the…

Q: Why the protien contamination happens during DNA isolation how the isolated DNA get contaminated by…

A: During the cycle of DNA isolation, the DNA test more often get contaminated with RNA and protein. To…

Q: Using the gel image: the three brightest fragments in the ladder are 100 bp, 500 bp, and 1000 bp in…

A: The gel electrophoresis is a method used to separate different fragments of DNA on the basis of…

Q: Which isotope did Hershey and Chase use to label bacteriophage nucleic acid? 32p 355

A: A. D. Hershey and Martha Chase, two geneticists, offered more proof in 1952. They experimented with…

Q: In which reagent is extracted DNA suspended to put it in solution? O Sodium dodecyl sulfate (SDS) O…

A: Introduction DNA extraction is a method to isolate DNA in a biological sample, which is done by…

Nutrient Growth Medium

It is a culture medium or nutrient broth for all kinds of microorganisms to grow properly and create their colony. This matrix provides support to increase the population of microbes through the method of cell proliferation. It is regarded as the food material for those organisms. It is a type of in vitro mechanism, in which the development of life is processed outside the host body. It can be solid, semi-solid, or liquid in nature. Different kinds of cells develop in different types of media.