Nicotinamide Adenine Dinucleotide (NAD) Assay (SIGMA Kit MAK037) analysis of tissue samples initially requires: centrifugation at 13,000g for 10min to homogenise tissue. centrifugation of 20mg of tissue at 2,000rpm with Extraction Buffer. freezing and thawing for 2 cycles of 20min before addition of Extraction Buffer. homogenization of PBS washed tissue with Extraction Buffer.

Basic Clinical Laboratory Techniques 6E
6th Edition
ISBN:9781133893943
Author:ESTRIDGE
Publisher:ESTRIDGE
Chapter2: Basic Hematology
Section2.3: Microhematocrit
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Nicotinamide Adenine Dinucleotide (NAD) Assay (SIGMA Kit MAK037) analysis of tissue samples initially requires:
centrifugation at 13,000g for 10min to homogenise tissue.
centrifugation of 20mg of tissue at 2,000rpm with Extraction Buffer.
freezing and thawing for 2 cycles of 20min before addition of Extraction Buffer.
homogenization of PBS washed tissue with Extraction Buffer.
Transcribed Image Text:Nicotinamide Adenine Dinucleotide (NAD) Assay (SIGMA Kit MAK037) analysis of tissue samples initially requires: centrifugation at 13,000g for 10min to homogenise tissue. centrifugation of 20mg of tissue at 2,000rpm with Extraction Buffer. freezing and thawing for 2 cycles of 20min before addition of Extraction Buffer. homogenization of PBS washed tissue with Extraction Buffer.
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