Among various dye-based assays like Biuret, Lowry, Bicinchronic Acid, and Biuret Protein Assays, what can be done if a concentrated sample is out of the detection range of chosen dye-based assay?
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Among various dye-based assays like Biuret, Lowry, Bicinchronic Acid, and Biuret Protein Assays, what can be done if a concentrated sample is out of the detection range of chosen dye-based assay?
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- If a concentrated sample is out of the detection range of a chosen dye-based assay, what can be done?what are the advantages and disadvantages of using recombinant protein and affinity chromatography for protein purification compared to gel filtration (size exclusion chromatography) and DEAE-sepharose chromatography (ion-exchange chromatography)?Why do we use standard solutions in protein assays? Explain briefly.
- For tha analysis of a specific drug present in different pharmaceutical preparations, explain when is more appropriate to use absorbtion based assay and when is more appropriate to use a fluorescence based assay.Why should there be 3 banding techniques (G, Q, and R)? Aside from their differences in appearance and procedure, what is unique to each one of them? or what can one banding technique show that the others cannot?IN A BIURET ASSAY TWO PROTEIN SOLUTION CONTAINING 150MG/ML AND 180MG/ML SHOWS THE SIMILAR ABSORBANCE. IS IT POSSIBLE?
- What is an ONPG test? WHat is he principle behind this assay.There are three names that often get used when discussing the Lowry assay. Those names are: Lowry, Folin-Ciocalteu, and Biuret. Are those names interchangeable when discussing the assay or do those names describe the same thing(s)? Significant differences? What is the relationship between those names?How is the Bicinchoninic acid assay used in protein quantification?