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- What is known as photoreactivation enzyme (PRE) or photolyase ?Define phototransduction.Briefly describe the pigments used in photosynthesis, where these pigments are located in the plant, and what these pigments do. Define both absorption spectrum and action spectrum, and describe the purpose of using a spectrophotometer to determine the absorption spectrum and how that correlates to the action spectrum. Be sure to discuss how the spectrophotometer allows the identification of the pigment solutions.
- Why is an isotonic buffered extraction medium used to isolate the chloroplasts? 1.To gently lyse the chloroplasts, leaving the electron transport membranes intact, whilst allowing DCPIP access 2.To maintain a constant acidic pH and favourable hyper-osmotic conditions, to ensure that the chloroplasts remained intact and functional during extraction 3.To gently separate the chloroplasts from each other, leaving the electron transport membranes intact, whilst allowing DCPIP access 4.To maintain a constant physiological pH and favourable iso-osmotic conditions, to ensure that the chloroplasts remained intact and functional during extraction.If a leaf containing the sameconcentration of chlorophyll as in the solution wasexposed to the same ultraviolet light, no fluorescencewould be seen. Propose an explanation for thedifference in fluorescence emission between thesolution and the leaf.To ensure that the plant was kept at a constant temperature at each light intensity in this experiment: Select one or more: a. the temperature of the room was adjusted to keep the tube of Elodea submerged in water at a constant temperature with a thermometer inside the tube b. the tube containing Elodea submerged in water was surrounded by light on all sides with a thermometer inside the tube c. the tube containing Elodea submerged in water was placed in a water bath with a thermometer in the water bath
- THE LIGHT DEPENDEN REACTION MUST OCCUR BEFORE THE LIGHT INDEPENDENT REACTION TRUE OR FALSE?Why do the reaction centers have different pigments rather than only the chlorophyls? quantitatively increasing the absorption photons narrowing coverage of the visible spectrum light increasing coverage of the visible spectrum light arranging light-harvesting complexe compensating for the high density of chlorophyllWhere does carbon dioxide fixation take place in the cellwith reference to the light-dependent reaction?
- To assess photosynthesis, we relied heavily on a spectrophotometer to measure the color intensity of DCPIP in our solution. We set the spectrophotometer at wavelengths in the visible spectrum. What is the approximate wavelength range (in nm) for the visible spectrum? For example, the ultraviolet spectrum wavelength range is approximately 10-400 nmWhen running cycle photophosphorylation and passaging electrons from PSI to two fully reduced PQH2s, how many photons would be necessary for the electrons to complete one cycle?: What is the difference between a fume hood and a biological safety cabinet (BSC)? Support your answer while considering HEPA filters. Where do fume hoods vent to?
