Place the following steps of the bacterial protein synthesis in their correct order? Peptide bond formation at the peptidyl-transferase center. Binding of MRNA and initiator formyl-methionyl-IRNAMet to the 30S ribosomal subunit. Aminoacylation and formylation of the initiator tRNAMet Joining of the 30S and the 50S ribosomal subunits Release Factor (RF) dependent hydrolysis of the peptidyl tRNA and release of the fully synthesized polypeptide from the ribosome Elongation Factor G (EF-G) dependent translocation of the nibosome by one codon along the MRNA

Place the following steps of the bacterial protein synthesis in their correct order? Peptide bond formation at the peptidyl-transferase center. Binding of MRNA and initiator formyl-methionyl-IRNAMet to the 30S ribosomal subunit. Aminoacylation and formylation of the initiator tRNAMet Joining of the 30S and the 50S ribosomal subunits Release Factor (RF) dependent hydrolysis of the peptidyl tRNA and release of the fully synthesized polypeptide from the ribosome Elongation Factor G (EF-G) dependent translocation of the nibosome by one codon along the MRNA

Biology 2e

2nd Edition

ISBN:9781947172517

Author:Matthew Douglas, Jung Choi, Mary Ann Clark

Publisher:Matthew Douglas, Jung Choi, Mary Ann Clark

Chapter15: Genes And Proteins

Section: Chapter Questions

Problem 3VCQ: Figure 15.16 Many antibiotics inhibit bacterial protein synthesis. For example, tetracycline blocks...

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All of the following are true about translation EXCEPT _____. as the ribosome moves from codon to codon, amino acids brought by successive tRNAs to the ribosome form a growing polypeptide when the ribosome reaches a stop codon, its subunits detach, and the mRNA and new polypeptide are released RNA polymerase assembles a strand of mRNA complementary to the coding strand of DNA Ribosomal subunits and a tRNA-carrying methionine converge on the start codon of an mRNA
Figure 15.16 Many antibiotics inhibit bacterial protein synthesis. For example, tetracycline blocks the A site on the bacterial ribosome, and chloramphenicol blocks peptidyl transfer. What specific effect would you expect each of these antibiotics to have on protein synthesis? Tetracycline would directly affect: tRNA binding to the ribosome ribosome assembly growth of the protein chain Chloramphenicol would directly affect tRNA binding to the ribosome ribosome assembly growth of the protein chain
Order the following choices to indicate the sequence of steps in the process of elongation of agrowing peptide chain. a. release of EF1-GDPb. EF2-GTP hydrolysis promotes translocation of the ribosomec. peptidyltransferase reaction catalyzed by large rRNA subunitd. aminoacyl-tRNA binds the ribosomal A site via EF1-GTP hydrolysis
Which of the following statements regarding O-linked glycosylation is FALSE? A. It occurs on asparagines (Asn) in specific proteins. B. It is an important post-translational protein modification. C. It is built sequentially on proteins by individual enzymes that are found in each cisterna of the Golgi (i.e. cis, medial, trans). D. It occurs in the Golgi. It occurs on serine (Ser) and threonine (Thr) residues in specific proteins.
For each amino acid incorporated into a polypeptide the cell has to invest three high-energy phosphates (ATP or GTP). At which step of the overall process is energy investment NOT required? What is the correct answer from below? Ester bond formation between a tRNA and an amino acid Release of an aminoacyl-tRNA into the A-site of a ribosome Translocation of a ribosome along an mRNA molecule Peptide bond formation between amino acids none of the above
Put the following events of elongation in prokaryotic translation in chronological order. 1. Binding of mRNA with small ribosomal subunit 2. Recognition of initiation codon 3. Complementary base pairing between initiator codon and anticodon of initiator tRNA 4. Base pairing of the mRNA codon following the initiator codon with its complementary tRNA 5. Attachment of the large subunit 2, 1, 4, 3, 5 5, 4, 3, 2, 1 1, 2, 3, 5, 4 1, 2, 3, 4, 5
Identify the following by describing their functions: EF-G, EF-Tu, EF-Ts, EF-P, and peptidyl transferase
Which of the following statements are true regarding the metabolome, the proteome, the genome, and the transcriptome? The number of genes found in the genome equals the number of different proteins that can be made within any give cell. The number of transcription products equals the number of proteins that can be made from these transcription products. The number of genes found in the genome equals the number of different transcription products that can be made within any given cell. The metabolome is encoded by the genome. None of the above.
Describe the process of ribosome synthesis in a bacterial cell by including each level of processing, any tRNA or rRNA transcript, ribosomal proteins, genome organization, RNA polymerase, relevant subunits of ribosomes name each subunit, related to large versus small subunit, each of rRNA, for example, 16S rRNA,
For each of the following, identify the type of RNA involved (mRNA, rRNA, or tRNA). a. Transports the correct amino acid to the ribosome, using the information encoded in the mRNA. b. Is a major component of ribosomes. c. Speciﬁes the order of amino acids in a protein, using a series of three-base codons, where different amino acids are speciﬁed by particular codons. d. Contains a three-base anticodon that pairs with a complementary codon revealed in the mRNA. e. Assists in making the bonds that link amino acids together to make a protein.
Antibiotic X binds to the 50S ribosomal subunit of 70S ribosomes and blocks normal ribosomal function. Which of the following is true of this drug? It would interfere with protein synthesis in bacteria, but not affect eukaryotic ribosomes. It would interfere with peptidoglycan syntheis/repair in bacteria, but not affect eukaryotes as they lack peptidoglycan. It would interfere with mRNA transcription in bacteria and in eukaryotes. It would interfere with protein synthesis in bacteria and eukaryotes. It would interfere with mRNA transcription in bacteria, but not affect eukaryotic transcription. It would interfere with peptidoglycan syntheis/repair in bacteria, as well as in eukaryotes that possess cell walls.
Which of the following are stages of translation? Select all that apply. ---A tRNA binds to the second codon and its carried amino acid forms a peptide bond with methionine. ---When the ribosome reaches a stop codon, its subunits detach, and the mRNA and new polypeptide are released. ---As the ribosome moves from codon to codon, amino acids brought by successive tRNAs to the ribosome form a growing polypeptide. ---The binding of a tRNA to the third codon causes the ribosome to release the first tRNA and move to the next codon. ---Ribosomal subunits and a tRNA carrying methionine converge on the start codon of an mRNA.