Part B. Identification of Mobile Phase for Separation of Acetanilide and Benzoic Acid Sketch your TLC plates. As before, measure all distances on the actual TLC plates, then record them on or adjacent to each sketch. 100% Pet. ether Std. Acetanilide R Std. Benzoic acid R O Mixture: Acetanilide R O Benzoic acid R 6 0 BC 100% ethyl acetate Std. Acetanilide R 040 Std. Benzoic acid R 0.57 3.1 (m Mixture: Acetanilide R 0-36 Benzoic acid R 0.59 7.69 cm A 44cm 14.50 28cm 50% Pet. ether / 50% ethyl acetate Std. Acetanilide R 0-20 Std. Benzoic acid R 047 Mixture: Acetanilide R 0.17 Benzoic acid R 0.44 7.5 CT 1.5cm A B 3.5cm 703 A- Acetanilide B-Benzoic Acid C-Acetanilid/Benzoic Which mobile phase provides the best overall results for the separation of acetanilide and benzoic acid? Explain why. 33cm 13 cm 7.5 cm

Fundamentals Of Analytical Chemistry
9th Edition
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Chapter33: High-performance Liquid Chromatography
Section: Chapter Questions
Problem 33.15QAP
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Would the answer to this question on the bottom be 100% Pet. ether? if so, why? if not, why not and which one is the best mobile phase and why? 
Part B. Identification of Mobile Phase for Separation of Acetanilide and Benzoic Acid
Sketch your TLC plates. As before, measure all distances on the actual TLC plates, then
record them on or adjacent to each sketch.
100% Pet. ether
Std. Acetanilide R 0
Std. Benzoic acid R O
Mixture:
Acetanilide R O
Benzoic acid R 6
0
BC
100% ethyl acetate
Std. Acetanilide R 0.40
Std. Benzoic acid R 0.57
3.1
(m
Mixture:
Acetanilide R 0-36
Benzoic acid R 0.59
7.63
cm
44cm
O
14.50
28cm
50% Pet. ether/ 50% ethyl
acetate
Std. Acetanilide R 0.20
Std. Benzoic acid Ry 0.47
Mixture:
Acetanilide R 0.17
Benzoic acid R 0.44
7.5
CTN
1.6cm
AB
3.5cm
A- Acetanilide
B-Benzoic Acid
C-Acetanilid/Benzoic
Which mobile phase provides the best overall results for the separation of acetanilide and
benzoic acid? Explain why.
33cm
13
cm
7.5
cm
Transcribed Image Text:Part B. Identification of Mobile Phase for Separation of Acetanilide and Benzoic Acid Sketch your TLC plates. As before, measure all distances on the actual TLC plates, then record them on or adjacent to each sketch. 100% Pet. ether Std. Acetanilide R 0 Std. Benzoic acid R O Mixture: Acetanilide R O Benzoic acid R 6 0 BC 100% ethyl acetate Std. Acetanilide R 0.40 Std. Benzoic acid R 0.57 3.1 (m Mixture: Acetanilide R 0-36 Benzoic acid R 0.59 7.63 cm 44cm O 14.50 28cm 50% Pet. ether/ 50% ethyl acetate Std. Acetanilide R 0.20 Std. Benzoic acid Ry 0.47 Mixture: Acetanilide R 0.17 Benzoic acid R 0.44 7.5 CTN 1.6cm AB 3.5cm A- Acetanilide B-Benzoic Acid C-Acetanilid/Benzoic Which mobile phase provides the best overall results for the separation of acetanilide and benzoic acid? Explain why. 33cm 13 cm 7.5 cm
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