please give calculation as well. Calculate the pH value of each of the solutions in tubes 1-9 using the Henderson-Hasselbalch equation (H-H eqn).Determine the pl of casein. Compare your experimental values with those found in the literature. Biomolecules. Thank you!

EBK A SMALL SCALE APPROACH TO ORGANIC L
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Chapter2: Crystallization
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please give calculation as well. Calculate the pH value of each of the solutions in tubes 1-9 using the Henderson-Hasselbalch equation (H-H eqn).Determine the pl of casein. Compare your experimental values with those found in the literature. Biomolecules. Thank you!

The protein is casein.

Table 5.1 Table of additions for the pl experiment
TUBE NUMBER
Additions (ml)
1
4
7.
8.
9.
Casein solution
1.00
1.00
1.00
1.00
1.00
1.00
1.00
1.00
1.00
Distilled H2O
8.40
7.75
8.75
8.50
8.00
7.00
5.00
1.00
7.40
0.01 M Acetic acid
0.60
1.25
0.1 M Acetic acid
0.25
0.50
1.00
2.00
4.00
8.00
1.0 M Acetic acid
1.6
Calculated pH
values (H-H eqn)
Actual pH values
5.81
5.11
4.90
4.66 4,43 4.45 3.91 3.54 3.29
3-
2)
Transcribed Image Text:Table 5.1 Table of additions for the pl experiment TUBE NUMBER Additions (ml) 1 4 7. 8. 9. Casein solution 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 Distilled H2O 8.40 7.75 8.75 8.50 8.00 7.00 5.00 1.00 7.40 0.01 M Acetic acid 0.60 1.25 0.1 M Acetic acid 0.25 0.50 1.00 2.00 4.00 8.00 1.0 M Acetic acid 1.6 Calculated pH values (H-H eqn) Actual pH values 5.81 5.11 4.90 4.66 4,43 4.45 3.91 3.54 3.29 3- 2)
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