Please help me answer how much volume of cell suspension needed (for 5*10^4 cells) in microliters. Pertinent Information shown in the image. Determine the number of cells you have per ml of your cell suspension.Number of cells counted in quadrant 1 =46Number of cells counted in quadrant 2 =38Number of cells counted in quadrant 3 =56Number of cells counted in quadrant 4 =38TOTAL number of cells for ALL 4 quadrants =178Number of cells per ml will equal:[(total # of cells counted) divided by 4] X 104 =,scientific notation)_445,000_cells/ml (useB. Setting up a new culture of cells1. You will need to determine the volume of your cell suspension needed to obtain 5 x 10^4 cells.Use the value for cells per ml you determined above in Part A.Example: If your cells per ml = 2 x 1055 x 104 cells= 0.25 ml, or 250 ul suspension2 x 105 cells/ml5 x 10^4 cells0.12235955= Volume of cell suspension needed:mLcells/mLVolume of cell suspension needed (for 5 x 104 cells):_microliters (uL)These cells will be used to prepare "protein lysate" for western blot.

For some experiments, it is essential to use the specified number of cells of microbes (or…

Answered: Please help me answer how much volume…

Please help me answer how much volume of cell suspension needed (for 5*10^4 cells) in microliters. Pertinent Information shown in the image

Basic Clinical Laboratory Techniques 6E

6th Edition

ISBN:9781133893943

Author:ESTRIDGE

Publisher:ESTRIDGE

Chapter2: Basic Hematology

Section2.4: The Hemacytometer

Problem 9RQ

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Concept explainers

Nutrient Growth Medium

It is a culture medium or nutrient broth for all kinds of microorganisms to grow properly and create their colony. This matrix provides support to increase the population of microbes through the method of cell proliferation. It is regarded as the food material for those organisms. It is a type of in vitro mechanism, in which the development of life is processed outside the host body. It can be solid, semi-solid, or liquid in nature. Different kinds of cells develop in different types of media.

Question

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Please help me answer how much volume of cell suspension needed (for 5*10^4 cells) in microliters. Pertinent Information shown in the image.

Determine the number of cells you have per ml of your cell suspension.
Number of cells counted in quadrant 1 =
46
Number of cells counted in quadrant 2 =
38
Number of cells counted in quadrant 3 =
56
Number of cells counted in quadrant 4 =
38
TOTAL number of cells for ALL 4 quadrants =
178
Number of cells per ml will equal:
[(total # of cells counted) divided by 4] X 104 =,
scientific notation)
_445,000_
cells/ml (use
B. Setting up a new culture of cells
1. You will need to determine the volume of your cell suspension needed to obtain 5 x 10^4 cells.
Use the value for cells per ml you determined above in Part A.
Example: If your cells per ml = 2 x 105
5 x 104 cells
= 0.25 ml, or 250 ul suspension
2 x 105 cells/ml
5 x 10^4 cells
0.12235955
= Volume of cell suspension needed:
mL
cells/mL
Volume of cell suspension needed (for 5 x 104 cells):
_microliters (uL)
These cells will be used to prepare "protein lysate" for western blot.

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