Please use this submission link to submit your flowcharts. You will need to submit a total of three flowcharts, one for each of your unknown microorganisms (gram positive rod, gram positive coccus, and gram negative rod). Please make sure that you only use the allowed tests: and microorganisms to complete your flowchart. You must identify each of the allowed organisms to a tip on the flowchart. Each organism must take no more than 3 total tests to completely identify
Please use this submission link to submit your flowcharts. You will need to submit a total of three flowcharts, one for each of your unknown microorganisms (gram positive rod, gram positive coccus, and gram negative rod). Please make sure that you only use the allowed tests: and microorganisms to complete your flowchart. You must identify each of the allowed organisms to a tip on the flowchart. Each organism must take no more than 3 total tests to completely identify
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POSSIBLE MICROBES & ALLOWED TESTS |
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MICROBE |
Gram rxn/shape |
Catalase Activity |
Mannitol/High Salt (growth/ferm) |
H2S prod. |
Lactose ferm./gas |
EMB (growth/pigment) |
MR /VP |
Indole |
Citrate |
Colony Color |
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Enterobacter aerogenes |
negative rod |
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- |
+ / + |
growth/pigment (pink fisheye) |
- / + |
- |
+ |
opaque |
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Escherichia coli |
negative rod |
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- |
+ / + |
growth/pigment (metallic green sheen) |
+ / - |
+ |
- |
opaque |
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Proteus vulgaris |
negative rod |
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+ |
- or wkA / - |
growth/no pigment |
+ / - |
+ |
- |
opaque |
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Salmonella typhimurium |
negative rod |
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+ |
- or wkA / - |
growth/no pigment |
+ / - |
- |
+ |
opaque |
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Pseudomonas fluorescens |
negative rod |
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- |
- or wkA / - |
+/- |
- / - |
- |
+ |
opaque |
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Micrococcus luteus |
positive cocus |
+ |
+ / - |
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- or wkA / - |
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gold |
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Staphylococcus aureus |
positive cocus |
+ |
+ / + |
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A / - |
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yellow |
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Staphylococcus epidermidis |
positive cocus |
+ |
+ / - |
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A / - |
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white |
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Streptococcus faecalis |
positive cocus |
- |
+ / + |
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A / - |
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opaque |
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Streptococcus lactis |
positive cocus |
- |
- / - |
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A / - |
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opaque |
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Interpretation notes: + = positive rxn; A= acid production; G=gas production; wk=weak + rxn; |
sl= slow + rxn |
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Incubation times are 24 hours if positive, 48 hours if negative at 24. |
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Test results obtained using small inoculum. |
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Always check for signs of growth before calling a test negative. You may have forgotten to inoculate it. |
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You may have the option of performing a confirming test if you are between two organisms for identification. |
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Check with your TA |
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