Given the scenario, compute for the total volume of the culture media solution (milliliter or liter) and dehydrated media (grams). Scenario: The students of a Microbiology class were tasked to transfer or subculture a pure culture of Escherichia coli bacterium in five 7 mL nutrient broth and five petri dishes of nutrient agar with 20 mL capacity each. Based on the instruction bottles for nutrient broth and nutrient agar, preparation of the culture media is as follows. Nutrient broth: 8 g/liter Nutrient agar: 28 g/liter   Formula: C1V1 = C2V2 *Concentration *Volume   Computation: What are the answers to the following. Weight in grams of nutrient broth: _________ Distilled water in mL for nutrient broth: __________ Weight in grams of nutrient agar __________ Distilled water in mL for nutrient agar: ____________

Microbiology for Surgical Technologists (MindTap Course List)
2nd Edition
ISBN:9781111306663
Author:Margaret Rodriguez, Paul Price
Publisher:Margaret Rodriguez, Paul Price
Chapter4: The Prokaryotes
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Given the scenario, compute for the total volume of the culture media solution (milliliter or liter) and dehydrated media (grams).

Scenario: The students of a Microbiology class were tasked to transfer or subculture a pure culture of Escherichia coli bacterium in five 7 mL nutrient broth and five petri dishes of nutrient agar with 20 mL capacity each.

Based on the instruction bottles for nutrient broth and nutrient agar, preparation of the culture media is as follows.

Nutrient broth: 8 g/liter

Nutrient agar: 28 g/liter

 

Formula: C1V1 = C2V2

*Concentration

*Volume

 

Computation:

What are the answers to the following.

  1. Weight in grams of nutrient broth: _________
  2. Distilled water in mL for nutrient broth: __________
  3. Weight in grams of nutrient agar __________
  4. Distilled water in mL for nutrient agar: ____________
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