Population calculation A culture of bacteria is started with an initial input of 250 cells. After 20 generations, how many bacteria would be present in the culture?
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- A culture with approximately 3x104 cells/mL were incubated. After 3 hours, the number of cells had increased to 6x105 a) How many generations have occurred?A species of bacteria has a doubling time of 24 hours. After incubating the initial population for one week, the final population was 6,400 cells. What was the initial population of that bacteria? Show all work. Equations: Nt = No x 2n Final concentration = (initial concentration)/(total dilution factor) Total dilution factor = (individual dilution factor)# of serial dilutionsANSWER TO QUESTION 6: 5.3x105 Using your answer from question 6, how many CFUs would be presented in 10 mL of the original culture?
- A culture with approximately 4x105 cells/mL were incubated. After 10 hours, the number of cells had increased to 5x109. a) How long was the generation time in minutes?b) How many generations have occurred?If there is a sample having 3.5x10^5 CFU/ml, and this cell count came from a sample of the original culture which had been diluted 1x10^-4 times, what is the number of CFU/ml of the original culture?A strep culture is sampled at two points in time, 1 hour and 10 minutes apart. 3.2 x 105 cells/ml are found in the first sample, and 1.8 x 107 cells/ml in the second sample. What was the generation or doubling time (in minutes)?
- Calculate the generation time in a growth experiment in which a medium was inoculated with 5 X 106 cells/mL of E. coli cells and following a 1 hour lag, grew exponentially for 5 hours, after which the population was 5.4 X 109cells/mLIn a serial dilution, you plate 1 ml of a 1/1000 dilution of a bacterial culture and find 25 colonies the next day. What was the concentration of cells in the original culture? A) 25 cells/ml B) 0.000025 cells/ml C) 25,000 cells/ml D) 26 cells/ml E) 0.025 cells/mlA medium was inoculated with 5 * 106 cells/ml of Escherichiacoli cells. Following a 1-h lag, the population grewexponentially for 5 h, after which the population was 5.4 * 109cells/ml. Calculate g and k for this growth experiment.
- In a viable plate count, each _________ represents a _________fromthe sample population.a. cell, colony b. colony, cell c. hour, generation d. cell, generationOriginal culture 50 colonies from .1mL, 1:10000 estimate the number of bacteria per unit volume in the original culture.If 1.0 mL of a 1:1000 dilution produces 200 colonies on a culture plate, how many bacteria were present per mL in the original (undiluted) sample? 200,000 20,000 2,000 200