Pre-laboratory Exercises 1. (a) Define Beer's law and give the mathematical equation (specify each symbol in the equation). Beer's Law states that the concentration of a chemical solution is directly proportional to its absorption of light & can be calculated in the following formula A= EbC ay-htercept (b) Briefly explain what Beer's plot is and how do you obtain the Beer's constant (k) from the plot. Beer's plot is a straight line with the absorptunty of zero & a slope of ab oreb. It represents Absorbance 8 can be calculate by multiplying Constant by the path length then dinding that value by the length 2. The following solutions are prepared from 0.200 MNICI, and their absorbance measured at 725 nm. Solution # 1 2 3 4 5 Volume (mL) of 0.200 MNICI 1.00 2.00 3.00 4.00 5.00 Volume (mL) of H-O 4.00 3.00 2.00 1.00 0.00 [NiCh] Absorbance at 725 nm (a) Calculate the molar concentration of NiCl₂ in each solution 1-5. 0.105 0.210 0.315 0.420 0.525

Appl Of Ms Excel In Analytical Chemistry
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Chapter4: Least-squares And Calibration Methods
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Experiment-A8: Spectrophotometry of Cobalt(
Pre-laboratory Exercises
1. (a) Define Beer's law and give the mathematical equation (specify each symbol in the equation).
concentration of achemical Solution
Beer's Law states that the
in the following
A = Ebc
is directly proportional to its absorption of light
& can be calculated
formula
(b) Briefly explain what Beer's plot is and how do you obtain the Beer's constant (k) from the plot.
is a straight line with _ay-intercept
Beer's plot
of zero & a slope of ab oreb. It represents
the absorptunity
the length
Absorbance 8 can be calculate by
multiplying
Constant by the path length then dinding that value by the
2. The following solutions are prepared from 0.200 MNICI, and their absorbance measured at 725 nm.
Solution #
1
2
3
4
5
Volume (mL) of
0.200 M NiCh
1.00
2.00
3.00
4.00
pride
5.00
Volume (mL)
of H₂O
4.00
3.00
2.00
1.00
0.00
INICI]
(a) Calculate the molar concentration of NiCl₂ in each solution 1-5.
Absorbance
at 725 nm
0.105
0.210
0.315
0.420
0.525
72
Transcribed Image Text:Experiment-A8: Spectrophotometry of Cobalt( Pre-laboratory Exercises 1. (a) Define Beer's law and give the mathematical equation (specify each symbol in the equation). concentration of achemical Solution Beer's Law states that the in the following A = Ebc is directly proportional to its absorption of light & can be calculated formula (b) Briefly explain what Beer's plot is and how do you obtain the Beer's constant (k) from the plot. is a straight line with _ay-intercept Beer's plot of zero & a slope of ab oreb. It represents the absorptunity the length Absorbance 8 can be calculate by multiplying Constant by the path length then dinding that value by the 2. The following solutions are prepared from 0.200 MNICI, and their absorbance measured at 725 nm. Solution # 1 2 3 4 5 Volume (mL) of 0.200 M NiCh 1.00 2.00 3.00 4.00 pride 5.00 Volume (mL) of H₂O 4.00 3.00 2.00 1.00 0.00 INICI] (a) Calculate the molar concentration of NiCl₂ in each solution 1-5. Absorbance at 725 nm 0.105 0.210 0.315 0.420 0.525 72
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