Prepare a graph showing the linear relationship between log function of molecular weight against Ve/Vo. The R2value must be 0.9 or higher. What is the linear equation from the standards given? What is the R2value?
Prepare a graph showing the linear relationship between log function of molecular weight against Ve/Vo. The R2value must be 0.9 or higher. What is the linear equation from the standards given? What is the R2value?
Principles of Instrumental Analysis
7th Edition
ISBN:9781305577213
Author:Douglas A. Skoog, F. James Holler, Stanley R. Crouch
Publisher:Douglas A. Skoog, F. James Holler, Stanley R. Crouch
Chapter26: An Introduction To Chromatographic Separations
Section: Chapter Questions
Problem 26.14QAP
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Question
Use the following information collected from a SIZE-EXCLUSION CHROMATOGRAPHY experiment to answer the questions below.
MW Standards |
Molecular Weight (kDa) |
Elution Volume (mL) |
blue dextran |
1000 |
85 |
myoglobin |
17.2 |
200 |
TIM |
53.3 |
178 |
hemoglobin |
62.2 |
156 |
IgG |
140 |
122 |
ATCase |
307.9 |
94 |
- Prepare a graph showing the linear relationship between log function of molecular weight against Ve/Vo. The R2value must be 0.9 or higher. What is the linear equation from the standards given? What is the R2value?
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