Prepare a graph showing the linear relationship between log function of molecular weight against  Ve/Vo. The R2value must be 0.9 or higher. What is the linear equation from the standards given? What is  the R2value?

Principles of Instrumental Analysis
7th Edition
ISBN:9781305577213
Author:Douglas A. Skoog, F. James Holler, Stanley R. Crouch
Publisher:Douglas A. Skoog, F. James Holler, Stanley R. Crouch
Chapter26: An Introduction To Chromatographic Separations
Section: Chapter Questions
Problem 26.14QAP
icon
Related questions
Question

Use the following information collected from a SIZE-EXCLUSION CHROMATOGRAPHY experiment to  answer the questions below. 

MW Standards 

Molecular Weight (kDa) 

Elution Volume (mL)

blue dextran 

1000 

85

myoglobin 

17.2 

200

TIM 

53.3 

178

hemoglobin 

62.2 

156

IgG 

140 

122

ATCase 

307.9 

94

  1. Prepare a graph showing the linear relationship between log function of molecular weight against  Ve/Vo. The R2value must be 0.9 or higher. What is the linear equation from the standards given? What is  the R2value? 
Expert Solution
steps

Step by step

Solved in 2 steps with 2 images

Blurred answer
Knowledge Booster
Statistics and Analytical Chemistry
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, chemistry and related others by exploring similar questions and additional content below.
Recommended textbooks for you
Principles of Instrumental Analysis
Principles of Instrumental Analysis
Chemistry
ISBN:
9781305577213
Author:
Douglas A. Skoog, F. James Holler, Stanley R. Crouch
Publisher:
Cengage Learning
Fundamentals Of Analytical Chemistry
Fundamentals Of Analytical Chemistry
Chemistry
ISBN:
9781285640686
Author:
Skoog
Publisher:
Cengage
Appl Of Ms Excel In Analytical Chemistry
Appl Of Ms Excel In Analytical Chemistry
Chemistry
ISBN:
9781285686691
Author:
Crouch
Publisher:
Cengage
EBK A SMALL SCALE APPROACH TO ORGANIC L
EBK A SMALL SCALE APPROACH TO ORGANIC L
Chemistry
ISBN:
9781305446021
Author:
Lampman
Publisher:
CENGAGE LEARNING - CONSIGNMENT