slide. The student used an eyepiece graticule to calculate the size of some of the onion cells. Figure 1 shows the student's eyepiece graticule and stage micrometer. The stage micrometer measures in millimetres. Figure 1 0 10 20 30 40 Eyepiece graticule 0 mm 2 Stage micrometer Use Figure 1 to calculate the size of one division on the student's eyepiece graticule, in micrometres. Show your working.
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- previous question? At higher magnifications, the millimeter markings may be too far apart to appear together in the field of view. If this happens, use the measurement from a lower magnification to make an estimate.For example, you can estimate the field of view diameter at 400x total magnification using the measured diameter at 100x total magnification. The magnification is 4 times greater so the field of view will be 4 times smaller. What is the field of view under the 40X objective of the microscope used. - The answer for this question was 3/4mmThe field of view (FOV) is the entire circular image we see when looking into the eyepiece. The diameter of the FOV gets smaller as we increase magnification. It can be measured by using a stage micrometer like a ruler, measuring from edge to edge. Notice that the stage micrometer is 1000 microns (µm) in length, and the field of view under the lowest magnification is 5000 µm. Describe how we measure it?Instruction: FILL IN THE BLANKS 1. Manipulate the ________________ knob to bring the objective about 1 to 2 mm away from the slide. Never look into the ____________ when bringing the objective close to the slide to avoid damaging or crushing the slide. A clear field of vision should be ___________ not gray. 2. In viewing objects under the microscope, it is observed that the microscope ______________ the direction of movement and _______________ the position of the image observed. 3. In shifting to higher magnification, simply rotate the _________________ to the desired objective. If the image is not clear manipulate the __________________ knob. 4. The total magnification of the specimen as seen in the microscope is 400x. This means that the ocular magnification is _______ x and the ______________ is 40x.
- My field is COSMETIC SCIENCE What information can be obtained from UV-vis Spectra and how this can be applied in your chosen field? (maximum of 500 words)Do the bottom diagram Find all th calculations Micrograph width is 0.2mm at 400x Draw a micrograph diagram and calculationsContrast how the views differ under high power (40x) and oil immerson (100x) objective lenses in terms of a) magnification b) detail of the cell, c) size of the area being viewed and d) resolution.
- his is the result of an experiment in a spectrophotometer. please explain the graph (red and green one)In absorption spectrophotometry: a. Absorbance is directly proportional to transmittance b. Percent transmittance is directly proportional to concentration c. Percent transmittance is directly proportional to the light path length d. Absorbance is directly proportional to concentrationWhich of the following is not required for a UV-vis absorption spectrophotometer? Group of answer choices camera detector monochromator light source
- 3. Using the information provided, calculate the size of the objects viewed. Hint you will need to calculate the FOV. These cells are being viewed under high power. Use the length of just one cell to estimate the number of cells that can fit into the FOV. Scanning power objective = 5X; Low power objective = 40X; High power objective = 100X; Eyepiece = 10X; Low power field of view (FOV) = 1.5 mmPrevious question: For example, you can estimate the field of view diameter at 400x total magnification using the measured diameter at 100x total magnification. The magnification is 4 times greater so the field of view will be 4 times smaller. What is the field of view under the 40X objective of the microscope. - The Answer I got for this question was 3/4mm.How will the following affect resolution during microscopy? I) Closing or opening the diaphragm II) Raising or lowering the condenser III) Increasing or reducing the light intensity