production, so you look for mutants that make higher amounts of the drug. You identify 16 high producing mutants. You mate haploid mutants 1-6 with haploid mutants 7-12 to produce diploid progeny and the amount of drug produced by the diploid progeny is shown in the Table below (Low indicates wild type quantities, High indicates about 100 times higher than wild type levels. Wild type 7 8 9 10 11 12 Wild type low high low low low low high 1 low high low low low low high 2 low high low high low low high 3 low high low high low low high low high high low high high high low high low high low low high low high low high low low high 5 6

production, so you look for mutants that make higher amounts of the drug. You identify 16 high producing mutants. You mate haploid mutants 1-6 with haploid mutants 7-12 to produce diploid progeny and the amount of drug produced by the diploid progeny is shown in the Table below (Low indicates wild type quantities, High indicates about 100 times higher than wild type levels. Wild type 7 8 9 10 11 12 Wild type low high low low low low high 1 low high low low low low high 2 low high low high low low high 3 low high low high low low high low high high low high high high low high low high low low high low high low high low low high 5 6

Name: Plz asap 2. You identify a fungus that produces low amounts of anew an
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Description: Plz asap 2. You identify a fungus that produces low amounts of anew anti-Covid-19 drug. You want to increase drugproduction, so you look for mutants that make higheramounts of the drug. You identify 16 high producingmutants. You mate haploid mutants

Biology: The Dynamic Science (MindTap Course List)

4th Edition

ISBN:9781305389892

Author:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Publisher:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Chapter18: Dna Technologies: Making And Using Genetically Altered Organisms, And Other Applications

Section: Chapter Questions

Problem 8TYK

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6. A diploid strain of yeast was made by mating a haploidstrain with a genotype w−, x−, y−, and z− with a haploidstrain of opposite mating type that is wild type for thesefour genes. The diploid strain was phenotypically wildtype. Four different X-ray-induced diploid mutantswith the following phenotypes were produced fromthis diploid yeast strain. Assume a single new mutation is present in each strain.Strain 1 w− x+ y− z+Strain 2 w+ x− y− z−Strain 3 w− x+ y− z−Strain 4 w− x+ y+ z+When these mutant diploid strains of yeast go throughmeiosis, each ascus is found to contain only two viablehaploid spores.a. What kind of mutations were induced by X-rays tomake the listed diploid strains?b. Why did two spores in each ascus die?
1. What do we need to consider in choosing the best method in introducing recombinant DNA? Compare and contrast at least three considerations (in terms of methods or requirements) in introducing recombinant DNA in plant calli vs animal cell lines. Answer this comprehensively and clearly.
7. Why recombinant DNA is very useful in improving our health conditions?A. Human insulin can be reproduced by bacteria?B. Vaccine can be reproduced by fungi or bacteriaC. Human growth hormone can be obtained from E. ColiD. All of the above 8. Which of the following is/are example of genetic engineering?A. A farmer choose a breed of cow for greater milk production.B. The use of bacteria in order to produce human insulinC. The insertion of clone genes to plant cellsD. Cross pollination of squash flowers. 9. Bacterial cells as host organisms may expose the gene to make protein products such as insulin and growth hormone . Which of the following processes are involved?A. replication and transcription. B. Transcription and replication C. Transcription and transformation. D. Replication and transformation
1. What are the considerations we need to consider in introducing recombinant DNA in plant calli and in animal cell lines? (Give at least three considerations) Explain and answer this comprehensively.
1. What are the considerations we need to consider in choosing methods in introducing recombinant DNA in plant calli vs in animal cell lines? (Give at least three considerations) Explain and answer this comprehensively.
1. How does site specific recombination differ from homologous recombination? a. It requires a small and defined sequence similarity between two DNA segments that will recombine. b. It requires a large sequence similarity between two DNA segments that will recombine. c. It involves exonucleases that will recognize the two DNA segments that will recombine. d. It involves endonucleases that will recognize the two DNA segments that will recombine. 2. What will be the consequence if the exonuclease domain of DNA polymerase III is mutated and rendered nonfunctional? a. The polymerase can still elongate the DNA template. b. Mutations due to replication errors will increase. c. There will be no synthesis of the lagging strand d. The primers in the lagging strand will not be removed. 3. Which of the following statements best explains the mechanism for DNA replication? a. It is reductive because half the total DNA present is copied. b. It is conservative because one resulting molecule…
1. Write if the statement is true and modify the statement with the correct answer if its false a. E. coli strains used in blue-white screening produce blue color complexes because their beta-galactosidases are activated via alpha-complementation. b. If you are a researcher trying to identify a gene that is responsible for a trait of interest, the first thing that you would do is to make a review of the literature that is available. c. The polylinker region in plasmids is where specific DNA sequences are cut by non-specific restriction enzymes. d. Blue-white screening is one of the last steps involved in determining if the gene of interest has been inserted successfully in plasmids.
1. A second site mutation within a single gene that reverts the gene back to its wild type phenotype Group of answer choices a. Intergenic Recombination b. Intragenic Recombination c. Intergenic Reversion d. Intragenic Reversion
5. A strain of Neurospora with genotype A B is crossed with a strain with the genotype a b. Half the progeny are A B and half are a b. Explain how this is possible?
1.) If Ethidium bromide was not added to a gel, what would happen? 2. How can you tell if an individual is heterozygous for the D1S80 marker?
1. Why is the relationship P=p2 used for homozygous loci?
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