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- Why should the ethanol/lysate boundary not be disturbed?Calculate the concentration of 0.2ml amino acid when path length is 1cm and has an absorbance value of 0.50, if a 0.5ml solution of same amino acid has a concentration of 0.72mg/ml with an absorbance of 0.9.Rx Of oxacinophtalmic solution 3% Disp. 10 mL How many milligrams of axacin contained in each milliter of the dispensed prescription?
- Determine the amount of glucose in the unknown sample by plotting a standard curve of Absorbance at 620 nm on Y-axis and μg of glucose on the x-axis.Calculate the quantity of glycerin needed to prepare 35-mL of the following ophthalmic solution.Erythromycin Lactobionate 500 mgDexamethasone Sodium Phosphate 100 mgGlycerin 2.5 mLSterile water for Injection, a.d. 100 mL Input answer as: (3 decimal places)____ mLThe isoelectric point of eIF4a is 5.02. Students are given a sample of cell lysate containing eIF4A at pH 7.4. They are also provided with two buffers of pH 7.4 to use for ion exchange chromatography. One buffer has a very high salt concentration (1 M), the other has a low salt concentration (0.1 M). Mariela decides to use an anion exchange column, while Ashok chooses a cation exchange column. Who made the better decision? Provide a detailed explanation of why one student will end up with a purer sample of eIF4A. Which buffer will you use while washing away impurities (high or low salt), and which would you use to remove eIF4A from the column? Explain your choice.
- What is the typical composition of Harris Hematoxylin Solution? Which cellular organelle was more visible after immersing the intestinal cells in hematoxylin?what is the concetration of a lysozyme solution with an absorbance of 0.720 measured at 280 nm( e= 36000 -1M -1cm,l=1cm)1.0 ml of serum albumin (BSA) solution was precisely diluted to 100 ml with a buffer solution, and the absorbance at 280 nm was measured from this buffer solution at a distance of 1 cm from the light. The result was A=1.0. BSA is 0.1%(=0.1g/l). The absorptivity is e(0.1%)=0.667, so 1g/l solution gives an absorbance of 0.667. What was the original protein content? Report in the result unit mg/ml with an accuracy of 0.1 mg.
- Infuse heparin at 1,200 units per hour from a solution containing 40,000 units of heparin in 500 mL D5W. How many mL/hr will deliver the ordered dosage?An IV bag of NS containing 30 g KCl is being administered at a rate of 80 mL/hr using a macrodrip tubing with a DF of 15 gtts/mL. How many drops per minute is that? Group of answer choices a. 30 gtts/min b. 22 gtts/min c. 10 gtts/min d. 20 gtts/minWhen comparing the biuret reaction to measuring protein absorbance directly at 280 nm, list two benefits of utilizing the biuret reaction to quantify protein concentration.