1. Histone methylation can have many different effects on gene expression. In some cases, histone methylation is associated with activation of transcription, whereas in other cases it can trigger the formation of heterochromatin and a decrease in transcription. If histone methylation has been detected in the region of gene YFG in yeast, describe an experiment that could distinguish whether the methylation is important to activate or repress transcription of gene YFG. al 6 2. An E. coli strain of chromosomal genotype lacl* lacP" lacO* lacz* lacY*. You wish to transform this strain into a wild-type lac operon by the addition of an extra piece of DNA (plasmid). a. What regulatory region(s), gene or genes would you add on this extra DNA that would make this strain express the lac operon genes as wild type? b. How would you design this plasmid if you want the strain of E. coli to express the lac operon genes even if lactose is absent in the medium (constitutively)?
1. Histone methylation can have many different effects on gene expression. In some cases, histone methylation is associated with activation of transcription, whereas in other cases it can trigger the formation of heterochromatin and a decrease in transcription. If histone methylation has been detected in the region of gene YFG in yeast, describe an experiment that could distinguish whether the methylation is important to activate or repress transcription of gene YFG. al 6 2. An E. coli strain of chromosomal genotype lacl* lacP" lacO* lacz* lacY*. You wish to transform this strain into a wild-type lac operon by the addition of an extra piece of DNA (plasmid). a. What regulatory region(s), gene or genes would you add on this extra DNA that would make this strain express the lac operon genes as wild type? b. How would you design this plasmid if you want the strain of E. coli to express the lac operon genes even if lactose is absent in the medium (constitutively)?
Biology 2e
2nd Edition
ISBN:9781947172517
Author:Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:Matthew Douglas, Jung Choi, Mary Ann Clark
Chapter16: Gene Expression
Section: Chapter Questions
Problem 14RQ: A scientist compares the promoter regions of two genes. Gene A’s core promoter plus proximal...
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Predict what would be the consequence on X inactivation of adding siRNAs that eliminate the product of the Jpx gene. Refer to both the role of Jpx and the expected result.
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