Question 9 Listen A tRNA with only the amino acid phenylanine (and no other amino acids) attached to it would be found at this site of the ribosome ○ A) E B) Z ○ C) P D) A
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GQ9
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- QUESTION 22 The DNA sequences that are most conserved between human and mouse would most likely be located in: A Highly-repeated sequences, such as microsatellite regions B Highly repeated sequences, such as Alu sequences C Moderately-repeated non-coding sequences D Coding regions of single-copy genesQuestion 1: tRNA and amino acyl tRNA synthetases Part g: What chemical and structural characters are shared by the amino acids Ile, Val and Met? Part h: What enzyme attaches Ile to tRNA? Part i: Compare the frequency of the correct Ile entering the active site and the frequency of an incorrect amino acid entering the active site of this enzyme. Be specific with numbers. Part j: Compare the frequency of the correct tRNA(Ile) entering the active site and the frequency of an incorrect tRNA entering the active site of this enzyme. Be specific with numbers. Part k: Apply the Entropy change formula and multiply by T = 303 K (30 C) to determine the energetic cost of accurately attaching Ile to its cognate tRNA(Ile). Part l: Is there enough energy in the chemical transformation of ATP → AMP + 2Pi to pay this information cost?QUESTION NO. 1 During initiation of protein synthesis, A. methionyl-tRNA appears at the A site of the 80S initiation complex. B. eIF3 and the 40S ribosomal subunit participate in forming a preinitiation complex . C. eIF2 is phosphorylated by GTP . D. the same methionyl-tRNA is used as is used during elongation . E. a complex of mRNA, 60S ribosomal subunit, and certain initiation factors is formed. QUESTION NO. 2 Normally, certain kinds of reiterated sequences occur in a chromosome as an interspersion pattern that is A. highly repetitive DNA sequences. B. the portion of DNA composed of single-copy DNA. C. Alu sequences. D. alcernacing blocks of single-copy DNA and moderately repetitive DNA. E. alternating blocks of short interspersed repeats and long interspersed repeats.
- Question 7: At third codon positions, approximately what proportion of muations are synonymous? A. None B. 4% C.30% D. 70%Question 1. Suppose that the diagram below represents the genomic organization of an enzyme involved in eye pigment production in mice. Within the gene are four exons. Biochemical analysis has revealed that the active site of the enzyme is located in the C terminus of the protein. The nucleotide length of each exon and intron is shown. The dinucleotide sequence GT represents the 5’ splice site and the dinucleotide sequence AG represents the 3’ splice site. Both the 5’ and the 3’ splice sites must be present for splicing to occur. Assume that the first and second stop codons are located immediately after the first and second 5’ splice sites, respectively; the third and fourth stop codons are located near the 3’ end of exons 3 and 4, respectively; all these stop codons are in the correct reading frame. E) Suppose you isolate a mutant mouse that has white eyes. When you examine the size of the eye pigment enzyme produced by this mouse, you see that it is 400 amino acids long. Sequence…Question 1: Your research project is concerned with the expression and purification of a protein called aldehyde dehydrogenase. Describe in detail all steps required from gene amplification to characterisation of the purified protein.
- QUESTION 46 Vancomycin and B-lactam antibiotics are similar in what way? OA. They both prevent NAM-NAG glycosdic bond formation in peptidoglycan OB. They both upregulate transpeptidase expression C. They both prevent peptide side chain cross-linking in peptidoglycan O D. They both contain beta lactam ring structures OE. They both cleave NAM-NAG glycosdic bonds in peptidoglycanQUESTION 12 The leucine zipper domain of transcription factors is not involved in DNA recognition but rather in facilitating dimerization. Given the chemical properties of the amino acid leucine, dimerization of transcription factors via this domain by (select the correct option). Facilitating hydrogen bonding with the aqueous environment. Chelation of bivalent ions such as Zn2+. Formation of coiled-coils through hydrophobic non-covalent interactions between evenly spaced Leu residues in alpha-helical domains. Physically connecting the two transcription factor subunits through unstructured loops.QUESTION 33 33. Which defines a codon? A. a protein that beins transcription by breaking apart H bonds B. a free-floating base that attaches to an open DNA strand C. the genetic code word of three bases on mRNA that specify one amino acid D. the strong bond between two complementary nitrogen bases QUESTION 34 34. What is the role of tRNA during translation? A. carry amino acids to the mRNA for correct placement into the protein chain B. carry ribosomes to the site of protein synthesis C. break apart mRNA and send it back to the nucleus so that it can be reused D. bond to open the DNA strand to carry the code for protein synthesis out of the nucleus QUESTION 35 35. Which of the following changes would be expected if a CAUUUG sequences of bases mutated to CACUUG? A. the amino acid sequence would be shorter than expected B. the identity of 1 amino acid would change C. the identity of more than 1 amino acid…
- QUESTION 23 Which of the following is not part of pre-mRNA processing in eukaryotes? Addition of a 5’ cap Excision of introns Addition of a 3’ poly-A tail Excision of the promoterQUESTION 16 Peptidyl transferase activity (peptide bond enzyme activity) is associated with what site in the ribosome? E P A XQUESTION 13 Where does transcription occur in eukaryotic cells? in the nucleus in the cytoplasm at the golgi apparatus at the plasma membrane
