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Question: Please help me explain why tubes 1 and 3 showed evidence of unhydrolyzed strach after 30 minutes. Procedure and data sheet are already given

Question: Please help me explain why tubes 1 and 3 showed evidence of unhydrolyzed strach after 30 minutes. Procedure and data sheet are already given

Biomedical Instrumentation Systems
Biomedical Instrumentation Systems
1st Edition
ISBN:9781133478294
Author:Chatterjee
Publisher:Chatterjee
Chapter15: Instrumentation In Medical Imaging
Section: Chapter Questions
Problem 8P
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Question: Please help me explain why tubes 1 and 3 showed evidence of unhydrolyzed strach after 30 minutes.

Procedure and data sheet are already given

Procedure: 1. Label 3 test tubes 1, 2 and 3. Place the substances in each test tube as indicated below: Tube 1% starch 0.1M NaCl Acid/base/water Saliva 1 10 ml 1 ml 1ml 0.05N HCI 2 ml 1 ml distilled H2O 2 10 ml 1ml 2 ml 1 ml 0.05N NaOH 3 10 ml 1 ml 2 ml 2. Mix well by shaking each tube and place in a water bath with temperature maintained at 40°C for 30 minutes. 3. Remove all tubes from the water bath and stir. 4. Perform iodine test by taking 1 drop from each tube and place in different holes of a spot plate. Add 1 drop iodine and mix. Note color. 5. Perform benedicts test. Benedict's – to 1 ml benedict's reagent, add 5 drops of the other half of each tube and heat in a boiling water bath for 3 minutes. Note color of precipitate. 6. If the result in iodine test is positive (violet to blue color) and the benedicts test is negative return the tubes in the water bath and wait for another 15 minutes. 7. Repeat procedure 4until the blue color of starch with iodine fail to appear. Note the time of the disappearance of the blue color in iodine test. 8. Then perform Benedict's test by placing 1 ml of benedicts reagent in a test tube and add 3 drops of the sample (that is already negative to iodine). Heat in a boiling water bath for 2 mins. Note color of precipitate formed. 9. If no brick red precipitate is formed, return the tube in the water bath (40°C) for 15 minutes. 10. Repeat procedures 8-9 until a brick red precipitate is obtained. Note time of the appearance of the precipitate.
Transcribed Image Text:Procedure: 1. Label 3 test tubes 1, 2 and 3. Place the substances in each test tube as indicated below: Tube 1% starch 0.1M NaCl Acid/base/water Saliva 1 10 ml 1 ml 1ml 0.05N HCI 2 ml 1 ml distilled H2O 2 10 ml 1ml 2 ml 1 ml 0.05N NaOH 3 10 ml 1 ml 2 ml 2. Mix well by shaking each tube and place in a water bath with temperature maintained at 40°C for 30 minutes. 3. Remove all tubes from the water bath and stir. 4. Perform iodine test by taking 1 drop from each tube and place in different holes of a spot plate. Add 1 drop iodine and mix. Note color. 5. Perform benedicts test. Benedict's – to 1 ml benedict's reagent, add 5 drops of the other half of each tube and heat in a boiling water bath for 3 minutes. Note color of precipitate. 6. If the result in iodine test is positive (violet to blue color) and the benedicts test is negative return the tubes in the water bath and wait for another 15 minutes. 7. Repeat procedure 4until the blue color of starch with iodine fail to appear. Note the time of the disappearance of the blue color in iodine test. 8. Then perform Benedict's test by placing 1 ml of benedicts reagent in a test tube and add 3 drops of the sample (that is already negative to iodine). Heat in a boiling water bath for 2 mins. Note color of precipitate formed. 9. If no brick red precipitate is formed, return the tube in the water bath (40°C) for 15 minutes. 10. Repeat procedures 8-9 until a brick red precipitate is obtained. Note time of the appearance of the precipitate.
EFFECT OF pH ON ENZYME ACTIVITY Results: Tube 1 Tube 2 Tube 3 Time in Minutes Iodine Benedict's Iodine Benedict's Iodine Benedict's (+) (orange-red precipitate) 30 + + (+) 15 (orange-red precipitate) + (+) + 15 (brick-red precipitate) (+) + 15 + (brick-red precipitate) Note: Mark (+) if the blue color appears and (-) if no blue color appears in iodine test. (+) if precipitate is formed and note the color of the ppt. and (-) if no precipitate is
Transcribed Image Text:EFFECT OF pH ON ENZYME ACTIVITY Results: Tube 1 Tube 2 Tube 3 Time in Minutes Iodine Benedict's Iodine Benedict's Iodine Benedict's (+) (orange-red precipitate) 30 + + (+) 15 (orange-red precipitate) + (+) + 15 (brick-red precipitate) (+) + 15 + (brick-red precipitate) Note: Mark (+) if the blue color appears and (-) if no blue color appears in iodine test. (+) if precipitate is formed and note the color of the ppt. and (-) if no precipitate is
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