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- When determining the clinical significance of cultures, a. the number of microbes is signifi cant. b. the presence of a single colony of a true pathogen may indicate the presence of the disease if the culture comes from a site known to be sterile (i.e., cerebrospinal fl uid). c. the repeated isolation of a relatively pure culture of any microorganism can mean it is an agent of disease, although this is not always the case. d. a range of tests may be needed to identify a pathogen. e. all of the above are true.What are the possible problems/limitations to be encountered in a laboratory experiment about the Isolation of Foodborne Pathogens on Selective, Differential, and Enriched media (e.g., blood agar, mcconkey, mannitol, XLT-4) using steak plating method. Provide a brief but concise explanation for every limitations, respectively.For each of the following aspects, compare and contrast the EnteroPluri-Test System to the API® 20E System for Enterobacteriaceae identification. a. time requirement b. specimen preparation c. tests utilized d. anaerobic conditions e. interpretation of results
- E1. A. Explain how the Snyder agar medium is both a selective and a differential medium. B. How is the differential nature of the medium used to detect susceptibility to dental caries?Below is a scenario requiring the use of laboratory equipment and instruments. A watery fecal sample is tested for the presence of Bacillus cereus and Vibrio cholerae. The sample is stored in a screw capped tube. You are tasked to grow the Bacillus in a solid medium while Vibrio in a liquid medium. Choices: A. Incubator, B Hanging Drop Slide , C.Petri Dish, D. Drying Oven, E. Bunsen Burner, F. Inoculating NeedlE , G. Volumetric Pipette, H. Screw-capped Tubes, I. Hot plate, J. Centrifuge, K. Inoculating Loop, L. Microscope, M. Beaker, N. Biosafety Cabinet 1. What could be used to determine if there are more bacteria in the sample aside from Bacillus and Vibrio? 2. What should you use if you want to observe the natural motility of Bacillus under a Brightfield microscope? 3. Where should you perform the task of sterilization and inoculation? 4.What should be used to transfer the sample to the broth? 5. What could be used to separate the solid from the liquid portion of the…Based on the journal article “Interpretation of the Tube Coagulase Test for Identification of Staphylococcus aureus”, what should be the result to conclude the organism is S.aureus? When is it necessary to have supplemental tests done for the identification of S.aureus?
- Make illustrated diagrams of stepwise procedure for the performance of the following tasks applying appropriate aseptic techniques. 2. Transfer of Broth Culture to Tubed Solid MediaAt the station there is a 2 mL microcentrifuge tube containing sheep blood and 3 tubes containing the following solutions: 0 mM, 300 mM, 600 mM of sucrose. The tubes are randomly labelled A, B, C. 2 mL of each solution were transferred to a different microcentrifuge tube, 20 ul of blood were then added to each tube, and they were mixed well by inverting the tube for multiple times. They were observed and it was determined whether the solution in each tube was hypotonic (Yes or No) relative to the blood cells. In addition, the solutions were diluted with water to help you determine which solution is which sucrose concentration. What sucrose concentration corresponds with each tube? Tube Hypotonic (Yes/No) Hypotonic after 1:1 dilution with water (Yes/No) Most likely Sucrose concentration (mM) A No Yes B Yes Yes C No No1. Illustrate in a diagram the identification scheme from gram-positive cocci.2. Discuss the principle involved in the biochemical tests in identifying species of staphylococci.3. Illustrate in a diagram the identification scheme for Streptococci.4. Discuss the principle involved in the biochemical tests in identifying species of Streptococci.
- Name two tests that are useful for the differentiation of pneumococci and oral viridans streptococci.What would be the expected out come of a triple sugar iron test If the bacteria is a pathogenic intestinal bacteria?What appropriate method should be used in controlling microbial growth in each of the following; chicken serum, fresh goat milk, nutrient broth, paracetamol and inoculation loop?