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Basic Clinical Laboratory Techniques 6E

Basic Clinical Laboratory Techniques 6E

6th Edition

ISBN:9781133893943

Author:ESTRIDGE

Publisher:ESTRIDGE

Chapter7: Basic Clinical Microbiology

Section7.4: The Gram Stain

Problem 2RQ

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A) Refer to the image below to answer this question. You have a bacterial culture with a
concentration of 4x10° bacterial cells per mL of broth. You borrow your initial culture tube
to a fellow scientist and she performs the following dilutions: From the original culture 1 mL
of broth was transferred to Flask A which contains 99 mL of sterile water. Then 1 mL from
Flask A is transferred to Flask B and then 1 mL from Flask B is transferred to Flask C.
buffer
1 ml
1 ml
1 ml
A
C
99 ml
99 ml
99 ml
CULTURE
Give the dilution of:
Flask A
Flask B
Flask C
0.1 ml
0.1 ml
Plate A
Plate B
Plate C
1.0 ml
1.0 ml
Determine the dilution factor for the following questions:
1. What is the dilution from the original to Flask A?
0.1 ml
1 ml
0.1 ml

7. 1 mL from Flask C is placed on plate C What is the dilution on plate C?
3. What is the dilution from the original to Flask B?
4. What is the dilution from Flask B to Flask C?
5. What is the dilution from the original to Flask C?
6. She then obtains 1 mL from Flask B that is placed on a new plate D (unfortunately not
labelled in the image). What is the dilution on plate D?

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