Q: Compare the process of polymerase chain reaction (PCR) to DNA replication. Discuss three…
A: PCR is in vitro process whereas DNA replication is in vivo process.
Q: Define the following terms:a. hypochromic effectb. DNA denaturationc. restriction endonucleasesd.…
A: Recombinant DNA is made by combining two or more DNA from other sources. The process depends on the…
Q: Selectable markers allow Select an answer and submit. For keyboard navigation, use the up/down arrow…
A: A selectable marker is a gene that is added into a cell, specifically in a bacterium or to cells in…
Q: Discuss three stages of TRANSFORMATION and RESTRICTION ANALYSIS OF PLASMID DNA experiment. What…
A: For transformation and restriction digestion analysis follow the given steps: 1. Cut the plasmid…
Q: Create a poster on the steps and other methods involved in recombinant DNA
A: Recombinant DNA technology is the latest and advanced technique in the molecular biology and…
Q: Which of the delivery techniques mentioned in Table 2 can be used to best transfer recombinant…
A: * They are many techniques for delivering the recombinant plasmids into the recipient cells. *…
Q: A restriction enzyme digests DNA into fragments. Name the technique used to check the progression of…
A: DNA and RNA are nucleic acids that are found in living cells. Almost all living cells contain both…
Q: Explain the role of each of the two enzymes used in the room temperature -PCR process. Reverse…
A: PCR ( Polymerase Chain Reaction ) is a techniques which makes millions or billions copies of a DNA…
Q: Define the following terms: Bacteriophage λ DNA Plasmid Map
A: Question - Define the following terms: Bacteriophage λ DNA Plasmid Map
Q: Use the gel to answer the following questions. You will be constructing a map of the plasmid,…
A: Plasmids have been used in genetic engineering for cloning and expression. These carry the genes for…
Q: The ____ plasmid contains genes for synthesizing connections between donor and recipient cells.
A: The plasmid is defined as a small, circular, extra-chromosomal, double-stranded DNA molecule present…
Q: 3000 bp 2000 bp 1000 bp 800 bp 700 bp 500 bp L Uncut EcoRI 1 BamHI Ncol EcoR1/BamH1 BamHI/Nco1…
A: Use the gel to answer the following questions. You will be constructing a map of the plasmid,…
Q: Explain the importance of primers in pinpointing the region of DNA to be amplified, and discuss why…
A: Note- According to our guidelines we have to answer only 1 question. So you can repost the other…
Q: To make the recombinant plasmid permeable to DNA molecules, which of the chemicals is added?a)…
A: The correct option is CaCl2 (b) CaCl2 is the chemical which is added to prevent degradation of the…
Q: state two ways one can extract an insert from a plasmid
A: a plasmid is typically a tiny extrachromosomal DNA molecule that is found within the cell and is…
Q: Which is used for cloning eukaryotic genes but not prokaryotic genes?a) Restriction enzymesb) DNA…
A: Restriction enzymes are the enzymes that cleave the deoxyribonucleic acid (DNA) at specific sites…
Q: Please answer this question in short but concise and cohesive way. "How would you select the host…
A: Recombinant DNA technology involves the introduction of a particular gene of interest into a host…
Q: Determine whether sample 1 and sample 2 are plasmid OR genomic DNA, justify your answer
A: By observing the bands of the two samples; we can say that sample 1: Lane 2: PBR3222 : A clear and…
Q: Use the gel to answer the following questions. You will be constructing a map of the plasmid,…
A: Restriction enzymes These are proteins that are isolated from the bacteria that cleave the sequences…
Q: Using the diagram below describe the significance of DNA sequencing for identification of mutations.
A: DNA sequencing is typically the process of determining the nucleic acid sequence the order of…
Q: Summarize plasmid purification and PCR product cleanup for later restriction digestion, paying…
A: Plasmids are extra-chromosomal DNA that are mostly observed in certain bacteria. They are circular…
Q: CRO-explain
A: Cro’s role in the CI–Cro bistable switch is critical for λ’s transition from lysogeny to lytic…
Q: Use the gel to answer the following questions. You will be constructing a map of the plasmid,…
A: Restriction mapping involves physically cuttings double stranded DNA by using restriction…
Q: .Describe the method you would use to test for the uptake of a plasmid with a DNA insert
A: Introduction: The introduction of recombinant DNA into a suitable host cell is followed by the…
Q: Using a flow diagram, elaborate on how you would generate a recombinant plasmid.
A: Plasmid is a circular double stranded DNA molecule found in bacteria and is capable of self…
Q: Restriction Enzymes with LongerRecognition Sites Produce Larger _______ Fragments
A: The restriction enzyme is the molecular scissors that cut at a specific site. These are naturally…
Q: nswer in following heading: What are plasmids ,What are vectors Plasmids in cloning and plasmids in…
A: Plasmids are most commonly used as cloning vectors in gene cloning. To make a recombinant DNA…
Q: Use the gel to answer the following questions. You will be constructing a map of the plasmid,…
A: Plasmids are circular double-stranded DNA elements. These are found in prokaryotes. The plasmids…
Q: Which statement is not correct regarding an R-plasmid? O A) An R-plasmid is a self-replicating ds…
A: Plasmids are extra chromosomal DNA. R plasmid is a type of plasmid found in bacteria .
Q: Replicate the DNA strand AAGGCTAACGGCATTTAACCC. Transcribe the DNA strand AAGGCTAACGGCATTTAACCC.…
A: In most organisms, genetic material is stored in the form of DNA. In humans, each cell's nucleus has…
Q: The result Jake obtained after transforming his competent Escherichia coli cells with plasmids is as…
A: Plasmid Bacterial cells and some eukaryotes have plasmid which is a small, circular, double-stranded…
Q: is shown below. Draw a restriction map of a plasmid with the appropriate restriction sites based on…
A: Restriction digestion is the fragmentation(cutting) of DNA on very specific sites (restriction…
Q: Demonstrate a procedure that you could adopt to restrict or digest the TOL plasmid.
A: Plasmids are extrachromosomal, circular, double-stranded, self-replicating DNA. They are naturally…
Q: Give the name of the enzyme that catalyzes each of the following reactions:(a) Makes a DNA strand…
A: Transcription is the first of several steps of DNA based gene expression in which a particular…
Q: Based on the image below, answer the following questions: Restriction enzymes PstI and ScaI were…
A: Restriction enzymes These enzymes have the capability to identify and cleave specific restriction…
Q: constructing a map of the plasmid, pDiddy. What is the smallest fragment size that the…
A: Double digestion Restriction enzymes are eye enzymes that induce cuts(cleavage) at their specific…
Q: Identify the component of the solution which is the extracted DNA
A: Everything living contains DNA. This DNA can be isolated for a variety of molecular biology…
Q: Describe how a plasmid can be genetically modified to include a piece of foreign DNA that alters the…
A: When it is cut, the gene of interest, or the desired gene, is added to the plasmid. Once the gene of…
Q: create a concept map illustrating the relationships among these key terms: 1. Foreign DNA; 2.…
A: Recombinant DNA technology is the combining of DNA molecules from two different organisms. This…
Q: Ligase enzymes are used to covalently bind together pieces of DNA during the process of cloning…
A: Ligases are a class of enzymes that promotes the binding of two DNA or RNA molecules. DNA ligase…
Q: What are plasmids and vectors how are they used in cloning and research? Write the answer in…
A: A plasmid is a small, circular, double-stranded DNA molecule that is distinct from a cell's…
Q: The steps that involve complementary base pairing is the second step in which the nucleotide is…
A: 1. DNA replication is the process by which two identical copies of the DNA template strand is…
Q: 30 The name of the small DNA fragment used to determine if the complementary sequence is present in…
A: The name of the small DNA fragment used to determine if the complementary sequence is present in…
Q: Part A. If student counts 63 colonies on their 10^-6 dilution LB plate. What was the original…
A:
Q: True or False: Plasmids can ONLY be used in transforming bacterial cells.
A: Plasmid is small , circular double stranded DNA that is different from chromosomal DNA of cell.…
Q: Briefly explain how an antibiotic-resistance gene and the lacZ gene can be used to determine which…
A: Plasmids have been used as cloning vectors in recombinant DNA technology. These plasmids carry genes…
Q: List three differences between a plasmid and a chromosome
A: Genetic material refers to nucleic acids that acts as a hereditary material. It is present in…
Second part asks: Explain the roles two of those enzymes would play in obtaining the recombinant plasmids
Step by step
Solved in 2 steps
- Why are antibiotic resistance markers such as ampR important components of bacterial plasmid cloning vectors? a. The plasmid must have resistance to accept DNA inserts. b. They allow the detection of plasmids that contain an inserted DNA fragment. c. They ensure the presence of the ori site. d. They ensure that the plasmid can be cut by a restriction enzyme. e. They allow identification of bacteria that have taken up a plasmid.1. What are the considerations we need to consider in introducing recombinant DNA in plant calli and in animal cell lines? (Give at least three considerations) Explain and answer this comprehensively.4. What is the name of the process by which bacteria pick up a different organism’s geneticmaterial?5. Genetically, how does the original bacterial DNA (plasmid) differ from the final bacterial DNAmolecule? (Do not say, “It is longer.”)6. Tetracycline is an antibiotic that is prescribed to kill bacterial infections. The transformedbacterial cell that you created has the tetracycline resistant gene in it. If this cell is placed on agrowth medium with tetracycline, will the bacteria grow or die? Explain.
- 1. Given the following restriction endonucleases and the sequences of their corresponding restriction sites, which would be LEAST useful for cutting the plasmid and the foreign DNA to be inserted? [The arrows indicate where cuts are made.] (see img) a. EcoRI b. HindIII c. HaeIII d. BamHI 2. EcoRI and HindIII are two different restriction enzymes. If the DNA from two different sources were cut with either EcoRI or HindIII, which of the cut DNA fragments would NOT join together easily so that they could be sealed with ligase? a. E. coli DNA cut with EcoRI and human DNA cut with HindIII b. E. coli DNA cut with HindIII and mouse DNA cut with HindIII c. human DNA cut with EcoRI and chimpanzee DNA cut with EcoRI d. mouse liver DNA cut with HindIII and mouse kidney DNA cut with HindIII1. What are the considerations we need to consider in choosing methods in introducing recombinant DNA in plant calli vs in animal cell lines? (Give at least three considerations) Explain and answer this comprehensively.What information and materials are needed to amplify region of DNA using PCR?
- Which of the following is not correct for bacterial conjugation? a. Both Hfr and F+ bacteria have the ability to code for a sex pilus. b. After an F- cell has conjugated with an F+, its plasmid holds the F+ factor. c. The recipient cell is Hfr following conjugation. d. In an HfrF- conjugation, DNA of the main chromosomemoves to a recipient cell. e. Genes on the F factor encode proteins of the sex pilus.The results of a paternity test using short tandem repeats are listed in the table below. Whos the daddy? How sure are you?1. What do we need to consider in choosing the best method in introducing recombinant DNA? Compare and contrast at least three considerations (in terms of methods or requirements) in introducing recombinant DNA in plant calli vs animal cell lines. Answer this comprehensively and clearly.
- 3. Using the same template strand, transcribe the DNA:GCA TAG CAA TGC5) Explain why phenol:chloroform is used in the isolation of genomic DNA ( What is the end result and what must you do for the desired effect1. As described in the textbook, the plasmid vector pBluescript II contains an ampicillin resistance gene and a lacZ gene, within which is located a multiple cloning site. Briefly describe how one selects for/identifies bacterial cells containing recombinant pBluescript II molecules and explain how the selection/identification works.