Streptococci/Enterococci Name of Organism: Enterococcus durans Type of Medium Blood Agar Color Before Inoculation Color After Incubation/Change in Medium Red Hemolysis: Zone of Inhibition: Bile Esculin Clear 6.5% Salt Broth Clear Name of Organism: Streptococcus salivarius Type of Medium Blood Agar Color Before Inoculation Color After Incubation/Change in Medium Red Hemolysis: Zone of Inhibition: Bile Esculin Clear 6.5% Salt Broth Clear
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- You are given a mixed culture of 3 bacteria–Methicillin-resistant Staphylococcus aureus(MRSA), Escherichia coli and Enterobacter aerogenes. Assume that Escherichia coli can ferment glucose, maltose, sucrose and lactose and also produce carbon dioxide gas on fermenting these. Assume that Enterobacter aerogenescan ferment only sucrose and cannot ferment glucose, maltoseand lactose. Also on fermenting sucrose it produces hydrogen sulphide gas. Assume that MRSA does not ferment any of these 4 sugarsbut forms endosporesand is sensitive to penicillin. Also they all give similar looking white-colored colonieson an agar plate. Question:Generate pure cultures of these 3 bacteria using the following tools that you learned in the lab. Use any (some or all)of these toolsto generate pure cultures as well as confirm them. A) Any of Staining methods B) Special media plates(McConkey Agar or Blood Agar or Mannitol Agar plates) C) Fermentation media tubes(Triple Sugar Iron (TSI)or Durham tubes) D) Any…Can you help me create a dichotomous key for these miccoorganisms and tests? Micoorganisms: Bacillus cereus Bacillus subtilis Citrobacter freundii Enterobacter aerogenes Enterococcus faecalis Escherichia coli Klebsiella pneumoniae Micrococcus luteus Mycobacterium phlei Proteus mirabilis Proteus vulgaris Pseudomonas aeruginosa Serratia marcescens Staphylococcus aureus Staphylococcus epidermidis Tests: Gram stain, acid-fast stain, endospore stain, amylase, urease, catalase, oxidase,carbohydrates (lactose, sucrose, glucose, mannitol, maltose), MR-VP, SIM, nitrate, motility,citrate, blood agar.In the tests MR-VP Test, Triple Sugar Iron test, and Blood Agar Plates (BAP) Streak-stab technique. What are the results for each test when looking at the bacteria: Micrococcus luteus Staphylococcus aureus Staphylococcus epidermidis Enterobacter aerogenes Escherichia coli Proteus vulgaris Pseudomonas aeruginosa Salmonella typhimurium Serratia marcescens
- SIM media Give me the expected results (3 each) for Enterobacter aerogenes, Salmonella typhimurium, and Escherichia coli. Enterobacter aerogenes- Salmonella typhimurium- Escherichia coli-Is colistin Naladixic acid agar selective and/or differential? ExplainBelow is a scenario requiring the use of laboratory equipment and instruments. A watery fecal sample is tested for the presence of Bacillus cereus and Vibrio cholerae. The sample is stored in a screw capped tube. You are tasked to grow the Bacillus in a solid medium while Vibrio in a liquid medium. Choices: A. Incubator, B Hanging Drop Slide , C.Petri Dish, D. Drying Oven, E. Bunsen Burner, F. Inoculating NeedlE , G. Volumetric Pipette, H. Screw-capped Tubes, I. Hot plate, J. Centrifuge, K. Inoculating Loop, L. Microscope, M. Beaker, N. Biosafety Cabinet 1. What could be used to determine if there are more bacteria in the sample aside from Bacillus and Vibrio? 2. What should you use if you want to observe the natural motility of Bacillus under a Brightfield microscope? 3. Where should you perform the task of sterilization and inoculation? 4.What should be used to transfer the sample to the broth? 5. What could be used to separate the solid from the liquid portion of the…
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- Can someone help me make a dichotomous key for for the species listed below Bacillus megaterium, Citrobacter freundii, Clostridium sporogenes, Enterobracter aerogenes, Escherichia coli, Proteus vulgaris, Pseudomonas aeruginosa, Salmonella enterica (Typhimurium), Serratia marcescens, Shigella sonnei Using these tests: TSA plates, Gram stain, catalase test, citrate slants, gelantinase tubes, oxidase test, PR Glucose broths, PR lactose Broths, PR sucrose broths, SIM tubes,Urease slants (not all but most)Identify a biochemical test that can differentiate Enterobacter agglomerans from Enterobacter cloacaeEPKP O111: K58 is isolated from the feces of a sick child under 3 months of age with certain clinical manifestations in the bacteriological laboratory. 1. What diagnosis can be given to a child? 2.What method of microbiological diagnosis was used in this case? Describe its main stages. 3. Is it possible to use serodiagnosis to verification of coli-enteritis? What material is taken from the patient for this purpose and what is used: glass-agglutination test, direct agglutination test or IHAT?