Substances A and B have retention times of 16.40 and 17.63 minutes, in a 30.0 cm column. A species that is not retained passes through the column in 1.30 minutes. The peak widths (at the base) for A and B are 1.11 and 1.21 minutes.Calculate:a) Resolution of the columnb) Average number of plates in the column.c) Height of the plate.d) Time required to elute substance B in a column that gives a resolution value, Rs = of 1.5.

Substances A and B have retention times of 16.40 and 17.63 minutes, in a 30.0 cm column. A species that is not retained passes through the column in 1.30 minutes. The peak widths (at the base) for A and B are 1.11 and 1.21 minutes.Calculate:a) Resolution of the columnb) Average number of plates in the column.c) Height of the plate.d) Time required to elute substance B in a column that gives a resolution value, Rs = of 1.5.

Fundamentals Of Analytical Chemistry

9th Edition

ISBN:9781285640686

Author:Skoog

Publisher:Skoog

Chapter31: Introduction To Analytical Separations

Section: Chapter Questions

Problem 31.32QAP

See similar textbooks

Similar questions

What are the properties that determine retention time in the Chromatography process? Please shortly write at your own words. (5-6 lines maximum ).
(b) For column 1(i) What are the unadjusted retention times for components A and B?
From the data calculate: a) retention factor of each solute b) selectivity factor c) how many plates are needed to provide a resolution of 1.5? d)how long a column is needed if the plate height of the packing is 1.5x10^-3cm? e)if the flow rate is 6.75 cm/min how long will it take to elite the two species?
Consider a 22.0 cm long column with a plate height of 0.0125 mm, a flow rate of 1.0 mL/min, and a Vm =1.2 mL (peak shapes are all symmetric). What is the solute retention time, retention volume, and peak width at half height when k’ = 7.2?
The three standards have different retention times on the Mini-GC. What physical characteristics of the molecules do you think contribute to this?
PART A: Substance A was found to have a retention times of 3.15 minutes on a 70.00-cm column. An unretained species passed through the column in 0.50 minutes. The peak width (at base) for A was 0.60 minutes. Using this information, determine the following: 1. What is the retention factor for analyte A? 2. What is the plate count for this column? 3. What is the height equivalent of a theoretical plate? PART B: Match each statement with one of these terms. A Partition coefficient B Mobile phase C Elution D Longitudinal diffusion E Stationary phase F Eddy diffusion G Retention factor H Plate number I Dead time J Retention time K Selectivity factor L Column resolution
A molecular exclusion column has a diameter of 7.8 mm and a length of 30 cm. The solid portion of the particles occupies 20% of the volume, the pores occupy 40%, and the volume between particles occupies 40%. (a) At what volume would totally excluded molecules be expected to emerge? (b) At what volume would the smallest molecules be expected? (c) A mixture of polymers of various molecular masses is eluted between 23 and 27 mL. What does this imply about the retention mechanism for these solutes on the column?
What is the role of specific visualizing agents in a Thin layer Chromatography? Please shortly write at your own words. Answer should be to the point (5-6 lines maximum ).
Chemistry What is the HETP of a column that is 200mm with a retention time of 5mins. The (effective) theoretical plate number N is 6000. What is the separation factor, a?
Describe how retention time of butanol on a poly (ethylene glycol) column will change with increasing temperature. Use the retention time for butanol in Figure 24-9b as the starting point.
Substance A and B were found to have retention times of 16.40 and 17.63 min. respectively, on a 30cm HPLC column. An unretained species passed through the column in 1.30 min. The peak widths at base for A and B were 1.11 and 1.21 min, respectively. Calculate the a.) Column resolution b.) the average nbumber of plates in the column and the plate height.
Two substances A and B have retention times of 16.40 and 17.63 min respectively on 30.0 cm column. An unsuccessful species passed over the column in 1.30 min. The widths (at the base) of the peaks ofA and B are respectively 1.11 min and 1.21 min: Calculate: a) The Resolution Rs of the column. b) The average number (Nav of theoretical plates in the column c) The equivalent height (H) d) length of column required to achieve a resolution of 1.5, e) time required to elute substance B on the longer column.