Sucrose is oxidized according to the following formula. C12H22O11 + O2 → CO2 + H2O You are investigating cellular metabolism in vitro. Under your experimental conditions, you plate 12 million cells in a dish using 10 ml of cell culture media. You grow the cells in a special instrument that can measure metabolic gases
Sucrose is oxidized according to the following formula.
C12H22O11 + O2 → CO2 + H2O
You are investigating cellular
plate 12 million cells in a dish using 10 ml of cell culture media. You grow the cells in a special
instrument that can measure metabolic gases. After 24 hours, you instrument has detected that
28.8 mg of oxygen have been consumed and the same mass of carbon dioxide released. Your
previous tests have shown that these cells consume 75 μg/106 cells-h.
(a) Determine the limiting reagent,
(b) percent excess of the other reagent, percent conversion for both reactants, and
(c)extent of reaction (ξ).
(d) Your initial concentration of sucrose in your cell culture media is 13.0mM. Your instrumentation can only resolve sucrose concentration at 0.1 mM increments. Can you detect the change in sucrose concentration with your instrumentation?
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