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- What is the difference between gram positive and gram negative bacteria? Describe how we use this to our advantage in the gram staining process. Hint: Gram positive versus Gram negative!!!A new lab recruit performs their first Gram stain on a slide containing both G(+) and G(-) organisms. After finishing the staining, they realize that they misread the labels on the crystal violet and Gram’s iodine vials. They accidentally used Gram’s iodine in place of crystal violet, and vice versa. Explain what you expect the recruit to see under the microscope, and explain why they see it.Give two reasons for the differential staining between gram + and gram - organisms
- what is the purpose of steaming the slide while applying malachite green in the endospore stain? a)To attach to the bacteria slide b)To create spores immediately c)To force the stain to penetrate the thick spore coat d)To ensure the bacterial cells are stained a red color e)To lyse the cell wall and kill the bacteriaYou use the Gram staining procedure to stain a bacterium that lacks a cell wall. Would you consider the bacterium appear Gram positive or Gram negative ? And why?Why is it important to perform a gram stain for any type of bacteria? After performing the gram stain method what would be the next step? (Think in terms of the real-world situation) Explain.
- Suppose you performed a Gram stain from a pure culture of bacteria and observed a field of red and purple bacilli. Adjacent cells were not always the same color. Provide an explanation for this observation.Why do you suppose endospore-forming bacteria are commonly found in the soil? Suppose your unknown bacteria is a Gram-negative rod. You decide to perform an endospore stain, thinking it will help you with the identification of the organism. What will you see, and why?An acid-staining method was performed on a bacterial smear using the dyes carboifuchsin and methylene blue. The resulting stained slide contained only cells that appeared blue when visualized using a microscope. Which of the following couid explain why only blue cells were present?