Suppose you cultured yeast, that you had transformed with pMZ379 plus the AVT5 sgRNA, on a plate of YES agar plus salt ions. You then cultured the same yeast on a plate containing only YES agar. Your results showed no growth on either plate. Which of the following is the most reasonable interpretation of your results? O A) The yeast were not transformed with pMZ379. B) The Cas9 gene was mutated, and was not able to cut the DNA and silence the yeast gene. C) The sgRNA gene was mutated in the plasmid, so that it did not lead the Cas9 to the correct yeast gene. D) The yeast were not viable or the conditions of growth in the experiment (temperature, humidity, etc.) were not sufficient to promote growth of the yeast. lecture 21.m4a Apr 20, 2022 19r

Suppose you cultured yeast, that you had transformed with pMZ379 plus the AVT5 sgRNA, on a plate of YES agar plus salt ions. You then cultured the same yeast on a plate containing only YES agar. Your results showed no growth on either plate. Which of the following is the most reasonable interpretation of your results? O A) The yeast were not transformed with pMZ379. B) The Cas9 gene was mutated, and was not able to cut the DNA and silence the yeast gene. C) The sgRNA gene was mutated in the plasmid, so that it did not lead the Cas9 to the correct yeast gene. D) The yeast were not viable or the conditions of growth in the experiment (temperature, humidity, etc.) were not sufficient to promote growth of the yeast. lecture 21.m4a Apr 20, 2022 19r

Biology: The Dynamic Science (MindTap Course List)

4th Edition

ISBN:9781305389892

Author:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Publisher:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Chapter17: Bacterial And Viral Genetics

Section: Chapter Questions

Problem 1ITD

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Austin Taylor and Edward Adelberg isolated some new strains of Hfr cells that they then used to map several genes in Escherichia coli by using interrupted conjugation. In one experiment, the researchers mixed cells of Hfr strain AB‑312, which were xyl+ mtl+ mal+ met+ and sensitive to phage T6, with F− strain AB‑531, which was xyl− mtl− mal− met− and resistant to phage T6. The cells were allowed to undergo conjugation. At regular intervals, the researchers removed a sample of cells and interrupted conjugation by killing the Hfr cells with phage T6. The F− cells, which were resistant to phage T6, survived and were then tested for the presence of genes transferred from the Hfr strain. The results of this experiment are shown in the graph. On the basis of these data, give the order of the xyl, mtl, mal, and met genes on the bacterial chromosome and the minimum distances between them in minutes. The origin of transfer is represented by the red triangle. The distances between genes are not…
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By conducting conjugation experiments between Hfr and recipientstrains, Wollman and Jacob mapped the order of many bacterialgenes. Throughout the course of their studies, they identified severaldifferent Hfr strains in which the F-factor DNA had been integratedat different places along the bacterial chromosome. A sample of theirexperimental results is shown in the following table:What information do you know based on the question and your understanding of the topic?
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