Nam- Number of Colonies Sdected sdeced That Grew On: Galatose - Cotransduction Galactose + Biotin Biotin 80 Gene Geme Frequency 10 palk" 0.125

Biochemistry
6th Edition
ISBN:9781305577206
Author:Reginald H. Garrett, Charles M. Grisham
Publisher:Reginald H. Garrett, Charles M. Grisham
Chapter30: Protein Synthesis
Section: Chapter Questions
Problem 8P
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In E. coli, the gene bioD+ encodes an enzyme involved in biotin synthesis, and galK+ encodes an enzyme involved in galactose utilization. An E. coli strain that contained wild-type versions of both genes was infected with P1 phage, and then a P1 lysate was obtained. This lysate was used to
transduce (infect) a strain that was bioD and galK. The cells
were plated on a medium containing galactose as the sole carbon
source for growth to select for transduction of the galK+ gene.
This medium also was supplemented with biotin. The resulting
colonies were then restreaked on a medium that lacked biotin to
see if the bioD+ gene had been cotransduced. The following results
were obtained:What information do you know based on
the question and your understanding of the topic?

Nam-
Number of Colonies
Sdected sdeced That Grew On:
Galatose - Cotransduction
Galactose + Biotin Biotin
80
Gene
Geme
Frequency
10
palk"
0.125
Transcribed Image Text:Nam- Number of Colonies Sdected sdeced That Grew On: Galatose - Cotransduction Galactose + Biotin Biotin 80 Gene Geme Frequency 10 palk" 0.125
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