Table 1- Comparison of the effect of catechol concentration on the amount of product formed. Absorbance Potato extract after 30mins (2nd reading) (mL) 1 Tube # la blank 2a 3a 4a 1 1 1 dH₂O Catechol (mL) (mL) 7 5 3 1 0 2 4 6 Absorbance 0 mins 1st reading 0.00 0.060 0.033 0-052 Q4) Give 2 reasons for adding dH₂O to these tubes in Table 1?. Time for reading: 3:21 -0.11 Q5) Tube la serves as a control, but why is this control needed? Absorbance: Time for reading: 3.36 Absorbance: 0.197 Time for reading: 3.37 Based on the data from Table 1 answer these questions: Q1) What is the name of the enzyme found in potato extract? Answer: Catechol Q2) What is the substrate? Answer: (10) Q3) Name of product of this enzyme catalyzed reaction? Answer: Absorbance: 0.152 Time for reading: 3:39 Absorbance: 0.66 Catechol Benzoquinone Subtract 1st from 2nd reading -0.0M 0.137 0.11.19 10.119 Q6) Notice that your 1st absorbance reading in tubes 2a-4a are quite similar but it then becomes very different at the 2nd absorbance reading? Explain why - hint you need to mention enzyme in your answer.
Table 1- Comparison of the effect of catechol concentration on the amount of product formed. Absorbance Potato extract after 30mins (2nd reading) (mL) 1 Tube # la blank 2a 3a 4a 1 1 1 dH₂O Catechol (mL) (mL) 7 5 3 1 0 2 4 6 Absorbance 0 mins 1st reading 0.00 0.060 0.033 0-052 Q4) Give 2 reasons for adding dH₂O to these tubes in Table 1?. Time for reading: 3:21 -0.11 Q5) Tube la serves as a control, but why is this control needed? Absorbance: Time for reading: 3.36 Absorbance: 0.197 Time for reading: 3.37 Based on the data from Table 1 answer these questions: Q1) What is the name of the enzyme found in potato extract? Answer: Catechol Q2) What is the substrate? Answer: (10) Q3) Name of product of this enzyme catalyzed reaction? Answer: Absorbance: 0.152 Time for reading: 3:39 Absorbance: 0.66 Catechol Benzoquinone Subtract 1st from 2nd reading -0.0M 0.137 0.11.19 10.119 Q6) Notice that your 1st absorbance reading in tubes 2a-4a are quite similar but it then becomes very different at the 2nd absorbance reading? Explain why - hint you need to mention enzyme in your answer.
Chapter21: Heparin Infusion Calculations
Section: Chapter Questions
Problem 1.3P
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