Temperature Exposures: Analyze the Results What temperatures were the most damaging? Why are the temperatures that you selected the most damaging? i.e. How do you know there is damage? Describe a possible mechanism that underlies the damage for each temperature selected. What temperatures were the least damaging or not damaging at all? How can you tell?
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- In sterilization, which among the supplies, instruments, glassware, etc. under the list of materials can be sterilized using either or both equipment below? List them down under the category: a) For “autoclaving” only, B) For Dry heat oven sterilization ,And C) Can be sterilized with either. Materials: 200-ml Erlenmeyer flask Stove 500-ml Erlenmeyer flask Autoclave 10-mL graduated cylinder Analytical balance 100-ml graduated cylinder pH meter Spatula Stirring rod 100-mL beaker Test tubes Distilled water Petri dish Stirring rod Alcohol lamp Glass dropperProve if the statement is TRUE or False with complete solution. At a dilution factor of, UHT-pasteurized milk sample X yielded the following bacterial colony counts when spread-plated in quadruplicates: 23, 20, 35, and 41 CFU/plate. As a result, the milk sample will pass laboratory standards if the total microbial limit is set to "not to exceed 30,000 CFU/mL of milk sample."What is the chemistry principle behind when (treatment: heavy cream) is subjected to room temperature? Why does beating time, stability (%drain) and volume foam (specific gravity) is important? see the data below Treatment (Heavy Cream) Beating Time (min) Stability (%drain) Volume of Foam (Specific Gravity) (Room Temperature) 20 No drain 0.94 Heavy Cream (chilled) 20 No drain 0.76 Heavy Cream with sugar and vanilla 20 No drain 0.88 Heavy Cream (over-whipped) 25 No drain 0.83
- Chemical Test Just fill out Table 2 by writing “+” if the compound is soluble/miscible in the solvent. Otherwise, write “-“. Table 2. Data on the results of chemical tests for C4H6O2. Chemical Test Observations + or - Ninhydrin test no colored spots on filter paper iodoform test clear pale-yellow solution Br2/CH2Cl2 (dark) clear red-orange solution Molisch test turbid colorless solution Tollen’s Test clear, colorless solution0.2 ml of culture . Please explain solutionSolution Absorbance mg/ml aspirin Standard solution - 1.6 mg/mL A 0.638 0.08 mg/mL B 0.504 0.064 mg/mL C 0.376 0.048 mg/mL D 0.259 0.032 mg/mL E 0.126 0.016 mg/mL A = -log T where T = %T ÷ 100 Construct a callibration curve using the above data. Absorbance should be on the vertical axis and "mg/mL of acetylsalicylic acid" on the horizontal axis. The line should go through the origin. Using the data provided, the graph you have generated, and the procedure that was used to generate the solutions which were examined by spectroscopy, calculate the amount of acetylsalicylic acid per tablet. Commercial tablet 1 labelled as 100 mg enteric coated Absorbance = 0.16 Commercial tablet 2 labelled as 300 mg Absorbance = 0.45 Student prepared tablet from practical 5 Absorbance = 0.19 Using the data provided, the graph you have generated, and the procedure that was used…
- Separation of Amino Acids by Thin Layer Chromatography Lab Questions 1. Describe in detail Thin layer chromatographic experiment. Example: the theory behind it, how youwould prepare the materials to spot on the plate with different mobile amino acids and unknown andhow TLC Plate is developed and the reasoning behind which solvent/ solvent mixture should be used,along how to correctly identify of the unknown. 2. Calculate the Rf value if a solute travelled 5 cm from the base spot and the solvent front is 10 cmfrom the origin? 3. In a TLC experiment using a 70:30 mixture of Petroleum ether and ethyl acetate, a student noted thedevelopment of spots in the origin, what can you suggest about this observation?A sample to be used for medical imaging is labeled with 18F,which has a half-life of 110 min. What percentage of the originalactivity in the sample remains after 300 min?Two compounds have Rf values of 0.50 and 0.61, respectively. The solvent front on a plate is developed to 10 cm. How far apart in centimeters are the spots for the two compounds?
- Answer the ff and state references: For his experiment, Brian needs to prepare 30 plates (use maximum volume), 15 slants (small test tube), and 15 stabs (big test tube) of Luria What is the total volume of the medium needed? What is the amount of each component needed, given the following media composition per liter of distilled water: Component g/L tryptone 10.0 NaCl 5.0 yeast extract 10.0 agar 15.0Laboratory Scenario Analysis. Read the following statements. Fill-in the possible effect of the procedure and give the rationale. Q: UV light in biosafety cabinet was left "turned on" while samples for product testing were being prepared.Effect:Rationale:Answer the ff with not more than two sentences: Why should Erlenmeyer flasks hold not more than 60% of their capacity? When can gelatin be used in culture media preparation? How does cotton plug prevent contamination of the medium? Why should caps of glassware be loosened first before sterilization?