The Effect of Various Factors on the Rate of an Enzyme Reaction. These experiments will use the enzyme catalase, which is found in potatoes. Catalase acts on the substrate, H2O2 (hydrogen peroxide), to produce H2O and O2. The O2 is released as bubbles. Therefore, the more bubbles you see, the greater the reaction. Base your responses on the following data table: The effect of temperature on the rate of enzyme reactions Tube Pre-Treatment Temp °C Treatment Temp °C Prediction - no bubbles + some bubbling ++ strong bubbling +++ very strong bubbling Observations - no bubbles + some bubbling ++ strong bubbling +++ very strong bubbling C1 100 100 +++ - C2 25 25 ++ +++ C3 0 4 + ++ What do you conclude about the effect of temperature on enzyme activity? Don’t just say that it has an effect, say how it affects it. Based on your conclusion, do you think you might digest ice cream at a slower or faster rate than a hot soup?

The Effect of Various Factors on the Rate of an Enzyme Reaction. These experiments will use the enzyme catalase, which is found in potatoes. Catalase acts on the substrate, H2O2 (hydrogen peroxide), to produce H2O and O2. The O2 is released as bubbles. Therefore, the more bubbles you see, the greater the reaction. Base your responses on the following data table: The effect of temperature on the rate of enzyme reactions Tube Pre-Treatment Temp °C Treatment Temp °C Prediction - no bubbles + some bubbling ++ strong bubbling +++ very strong bubbling Observations - no bubbles + some bubbling ++ strong bubbling +++ very strong bubbling C1 100 100 +++ - C2 25 25 ++ +++ C3 0 4 + ++ What do you conclude about the effect of temperature on enzyme activity? Don’t just say that it has an effect, say how it affects it. Based on your conclusion, do you think you might digest ice cream at a slower or faster rate than a hot soup?

Biology Today and Tomorrow without Physiology (MindTap Course List)

5th Edition

ISBN:9781305117396

Author:Cecie Starr, Christine Evers, Lisa Starr

Publisher:Cecie Starr, Christine Evers, Lisa Starr

Chapter4: Energy And Metabolism

Section: Chapter Questions

Problem 4SQ

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The Effect of Various Factors on the Rate of an Enzyme Reaction. These experiments will use the enzyme catalase, which is found in potatoes. Catalase acts on the substrate, H2O2 (hydrogen peroxide), to produce H2O and O2. The O2 is released as bubbles. Therefore, the more bubbles you see, the greater the reaction. Base your responses on the following data table: The effect of pH on the rate of enzyme reactions Tube pH Prediction - no bubbles + some bubbling ++ strong bubbling +++ very strong bubbling Observation - no bubbles + some bubbling ++ strong bubbling +++ very strong bubbling 1 3 - - 2 5 - - 3 7 + ++ 4 9 - +++ 5 11 - + Relate this to protein structure and denaturization. Again, thinking of the effect of pH on enzyme activity, why does the pancreas secrete HCO3-? What would you predict to happen to digestion and absorption in the small intestine if the pancreas stopped producing bicarbonate?
The rate of a chemical reaction will double (reaction will go twice as fast) for every 10°C increase in temperature. This observation holds for enzyme-catalyzed reactions up to a certain temperature range (different for each enzyme), at which the rate stops increasing and then decreases sharply as the temperature increases. Explain.
An introduction of the effects of subtract concentration on enzymes.
Many mammals including cattle and pigs have temperature close to 37 degree Centigrade. Can do you predict, why some enzymes of these animals are most active close 37 degrees Centigrade?
how reaction rate of enzyme-catalyzed reactions are affected by substrate and enzyme concentration, temperature, and pH
Comparing the enzymes in dishwashing detergent to the enzymes in your digestive system what are some similarities and differences neither for enzyme function at their best in these environments
Which of the following statements is NOT true? None of the other four answers (all are true) The energy required to cause a chemical reaction to occur is called the activation energy Decomposition (degradation, catabolic) reactions are usually endergonic Chemical reactions in living cells are catalyzed by enzymes Enzymes increase the probability that a chemical reaction will take place by lowering the activation energy
Energy Transformations How energy is transformed in animal and plant cells for use in cellular activities (use the following keywords, food, light, NADH, FADH2, NADPH, electron transport chain, ATP Synthase and ATP).
The Krebs cycle and why enzymes are not needed to power it and how enzymes can be affected by temperature, specfically, amylase in human saliva
What does it require for the necessary reactions to occur if there is the absence of enzymes? choices: higher temp, larger cells, smaller atoms, larger proteins
The reason for the increase in the rate of an enzyme reaction when the temperature is increased isa. decrease in kinetic energy of reactantb. increase in kinetic energy of reactantc. increase in activation energy of reactiond. decrease in activation energy of reaction
Enzymes work by lowering the activation energy needed to start biochemical reactions and increase the reaction rate. However, increasing the reaction temperature can also help to increase the reaction rate and speed up the reaction. In your opinion, does this mean all enzymes can be operated at an extreme reaction rate at an extremely high temperature?

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Tube	Pre-Treatment Temp °C	Treatment Temp °C	Prediction - no bubbles + some bubbling ++ strong bubbling +++ very strong bubbling	Observations - no bubbles + some bubbling ++ strong bubbling +++ very strong bubbling
C1	100	100	+++	-
C2	25	25	++	+++
C3	0	4	+	++