The Effect of Various Factors on the Rate of an Enzyme Reaction. These experiments will use the enzyme catalase, which is found in potatoes. Catalase acts on the substrate, H2O2 (hydrogen peroxide), to produce H2O and O2. The O2 is released as bubbles. Therefore, the more bubbles you see, the greater the reaction. Base your responses on the following data table: The effect of pH on the rate of enzyme reactions Tube pH Prediction - no bubbles + some bubbling ++ strong bubbling +++ very strong bubbling Observation - no bubbles + some bubbling ++ strong bubbling +++ very strong bubbling 1 3 - - 2 5 - - 3 7 + ++ 4 9 - +++ 5 11 - +   Relate this to protein structure and denaturization. Again, thinking of the effect of pH on enzyme activity, why does the pancreas secrete HCO3-? What would you predict to happen to digestion and absorption in the small intestine if the pancreas stopped producing bicarbonate?

Biology Today and Tomorrow without Physiology (MindTap Course List)
5th Edition
ISBN:9781305117396
Author:Cecie Starr, Christine Evers, Lisa Starr
Publisher:Cecie Starr, Christine Evers, Lisa Starr
Chapter4: Energy And Metabolism
Section: Chapter Questions
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  1. The Effect of Various Factors on the Rate of an Enzyme Reaction. These experiments will use the enzyme catalase, which is found in potatoes. Catalase acts on the substrate, H2O2 (hydrogen peroxide), to produce H2O and O2. The O2 is released as bubbles. Therefore, the more bubbles you see, the greater the reaction.

Base your responses on the following data table:

The effect of pH on the rate of enzyme reactions

Tube

pH

Prediction

- no bubbles

+ some bubbling

++ strong bubbling

+++ very strong bubbling

Observation

- no bubbles

+ some bubbling

++ strong bubbling

+++ very strong bubbling

1

3

-

-

2

5

-

-

3

7

+

++

4

9

-

+++

5

11

-

+

 

  1. Relate this to protein structure and denaturization. Again, thinking of the effect of pH on enzyme activity, why does the pancreas secrete HCO3-?
  2. What would you predict to happen to digestion and absorption in the small intestine if the pancreas stopped producing bicarbonate?
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