The H295R cells for the final experiment were treated with Atrazine using the following protocol: Step 1: Combine 1 μL of (3 X 10³ ppm Atrazine, DMSO) 0.999 ml of cell culture media Step 2: Combine the following 3 liquids (consider the final volume of this mixture = 60 ml for your calculations). 60 μl of the solution prepared in step 3 ml of cells suspended in cell culture media. 57 ml of cell culture media d. What is the concentration of DMSO in the treated cells in %v/v units?

Biomedical Instrumentation Systems
1st Edition
ISBN:9781133478294
Author:Chatterjee
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Chapter5: Biomedical Electronics: Digital
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3d
The H295R cells for the final experiment were treated with Atrazine using the
following protocol:
Step 1: Combine
1 µl of (3 X 10 ppm Atrazine, DMSO)
0.999 ml of cell culture media
Step 2: Combine the following 3 liquids (consider the final volume of this mixture
= 60 ml for your calculations).
60 µl of the solution prepared in step 1
3 ml of cells suspended in cell culture media.
57 ml of cell culture media
d. What is thẻ concentration of DMSO in the treated cells in %v/v units?
Transcribed Image Text:The H295R cells for the final experiment were treated with Atrazine using the following protocol: Step 1: Combine 1 µl of (3 X 10 ppm Atrazine, DMSO) 0.999 ml of cell culture media Step 2: Combine the following 3 liquids (consider the final volume of this mixture = 60 ml for your calculations). 60 µl of the solution prepared in step 1 3 ml of cells suspended in cell culture media. 57 ml of cell culture media d. What is thẻ concentration of DMSO in the treated cells in %v/v units?
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