The H295R cells for the final experiment were treated with Atrazine using the following protocol: Step 1: Combine 1 μL of (3 X 10³ ppm Atrazine, DMSO) 0.999 ml of cell culture media Step 2: Combine the following 3 liquids (consider the final volume of this mixture = 60 ml for your calculations). 60 μl of the solution prepared in step 3 ml of cells suspended in cell culture media. 57 ml of cell culture media d. What is the concentration of DMSO in the treated cells in %v/v units?
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- For the serial dilution, your stock solution must have a concentration of 3.5 mg/mL. How much diluent must be added to the 5.3 mg/mL red cell to prepare the stock solution? Show pertinent solution/s. 7. If the red cell suspension is the stock solution, what is being quantified in this test? What diluent/reactant should be used to detect your answer in number 7?What mode of chromatographic separation (RPLC, NPLC, HILIC, SEC, IEX) is best used for the specified Experiment Analysis of monosodium glutamate from Beef Broth Cubes, including those of other ions like NaCl? What stationary phase and mobile phase (together with elution technique), and other chromatographic conditions needed?Imagine you will use a centrifuge in the laboratory indicating rpm and your protocol requires 3500xg, what would your setting be in a given centrifuge with a radius of 10cm? Provide computations
- For the serial dilution, your stock solution must have a concentration of 3.5 mg/mL. How much diluent must be added to the 5.3 mg/mL red cell to prepare the stock solution? Show pertinent solution/s. What are the initial concentrations used for tubes 3, 5, and 6? Show pertinent solutions. What are the dilutions of the last positive tube and first negative tube respectively? Show computation.Using conversion factors, solve each of the following clinicalproblems:a. A nurse practitioner prepares an injection of promethazine,an antihistamine used to treat allergic rhinitis. If thestock bottle is labeled 25 mg /mL and the order is a doseof 12.5 mg, how many milliliters will the nurse draw up inthe syringe?b. You are to give ampicillin 25 mg/kg to a child with a massof 62 lb. If stock on hand is 250 mg /capsule, how manycapsules should be given?Why does eluent strength increase as solvent becomesless polar in reversed-phase chromatography, whereas eluentstrength increases as solvent becomes more polar in normal-phase chromatography? What kind of gradient is used in supercritical fluid chromatography?
- Describe various detection methods used in High-Performance Liquid Chromatography (HPLC) and types of biomolecules that are detected in each method?) In urea assay (Catalog #K375-100) what is the nmol (range) it can detect?Why is control of column and detector temperature more important for non- suppressed IC (Ion Chromatography) than it is for suppressed IC?
- Using conversion factors, solve each of the following clinical problems:a. The physician has ordered 1.0 g of tetracycline to be given every six hours to a patient. If your stock on hand is 500-mg tablets, how many will you need for one day’s treatment?b. An intramuscular medication is given at 5.00 mg / kg of body weight. What is the dose for a 180-lb patient?c. A physician has ordered 0.50 mg of atropine, intramuscularly. If atropine were available as 0.10 mg /mL of solution, how many milliliters would you need to give?d. During surgery, a patient receives 5.0 pt of plasma. How many milliliters of plasma were given?200 ml of a 2% protein solution are available, containing an enzyme to be purified. Half of the sample is subjected to method A, consisting of fractionated precipitations, and 5 ml of final solution are obtained, with a protein concentration equal to 5 mg / ml and enzymatic activity equal to 2000 U / ml. The other half is subjected to method B, consisting of ion exchange chromatography, and a final solution of 10 ml is obtained, with a protein richness equal to 10 mg / ml, and with an enzymatic activity equal to 2000 U / ml. You want to know: a) Which of the methods has provided the purest enzyme. b) By which of the methods has the greatest amount of protein been obtained.Name two radioisotopes used within the medical imaging processes, one administered by inhalation and one used by intravenous injection. For both of these radioisotopes, what are their half-life and what is the relevance of that to why it is used? What are both of these specific use in diagnostic imaging?What are they treating?