In urea assay (Catalog #K375-100) what is the nmol (range) it can detect?
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) In urea assay (Catalog #K375-100) what is the nmol (range) it can detect?
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- In nickel column affinity chromatography,how does the protein binds to column?How is the protein eluted from the column?In a gel filtration chromatography, what type of gel must be used when the protein size is 2500 Da? Explain.For paper chromatography, the obtained Rf value of an amino acid with uncharged nonpolar R group is greater than the obtained Rf value of an amino acid with charged polar R goup. TRUE OR FALSE?
- What is the difference of measuring proteins through UV-VIS spectroscopy at 280 nm and at 595 nm with the presence of the Bradford reagent? (note: explain not less than 5 sentences).Five amino acids were subjected to reverse phase chromatography, and the following Rf values were computed: A - 0.23 B - 0.08 C - 0.54 D - 0.74 E - 0.96 Which is the most polar amino acid?Five amino acids were subjected to normal phase chromatography, and the following Rf values were computed: A - 0.59 B - 0.12 C - 0.71 D - 0.33 E - 0.20 Which is the most polar amino acid?
- What are the components of the buffers used for protein hydrophobic column chromatography and explain how do they work?For the following sequence, what is the approximate annealing temperature? 5'-AGCTACGATCAGGTCA-3'Which nuclear isotope used in protein NMR spectroscopy is the most sensitive to detect? Briefly explain why.