The illustration below shows the lac operon used in E. coli. Explain what is happening in the bottom picture. The inducer is binding to the repressor, rendering it inactive and turning the operon on. Lactose is binding to the repressor, turning the operon off. Lactose is binding to the repressor, rendering it inactive and turning the operon off. O The operon, which is usually off, is turned on by the presence of glucose.

The illustration below shows the lac operon used in E. coli. Explain what is happening in the bottom picture. The inducer is binding to the repressor, rendering it inactive and turning the operon on. Lactose is binding to the repressor, turning the operon off. Lactose is binding to the repressor, rendering it inactive and turning the operon off. O The operon, which is usually off, is turned on by the presence of glucose.

Biology (MindTap Course List)

11th Edition

ISBN:9781337392938

Author:Eldra Solomon, Charles Martin, Diana W. Martin, Linda R. Berg

Publisher:Eldra Solomon, Charles Martin, Diana W. Martin, Linda R. Berg

Chapter14: Gene Regulation

Section: Chapter Questions

Problem 2TYU

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You are growing E. coli in a laboratory in order to study their operons. The growth media you are using contains lactose, no glucose and no tryptophan. Using your knowledge of operons and their regulation, answer the following questions using the dropdown menus provided in Canvas. Which operons would be functional under these conditions? What repressors would be made? (NOTE: name the repressors using their gene names) Which repressor(s) would be made in the inactive form? Which repressor(s) would be made in the active form? Which repressor(s) under these conditions can bind the operator sequence? Which repressor(s)under these conditions cannot bind the operator sequence? What effect does lactose have on the bacterial cell’s lac operon? What does the absence of glucose do to the bacterial cell? You now add tryptophan to the cell. What would happen to the bacterial cell and its trp operon? What kind of regulation does lactose provide to an operon’s…
In the tryptophan operon of E. coli, what happens when tryptophan levels get too high? tryptophan is removed from its co-repressor, causing it to detach from the operator tryptophan is removed from its co-repressor, causing it to attach to the operator tryptophan is bound to its co-repressor, causing it to detach from the operator tryptophan is bound to its co-repressor, causing it to attach to the operator all of the above occur at equal rates when tryptophan levels get too high (Option C is the answer for the question above) What effect will the above process have on the tryptophan operon? RNA polymerase will detach from the promoter, initiating transcription RNA polymerase will detach from the promoter, stopping transcription RNA polymerase will attach to the promoter, initiating transcription RNA polymerase will attach to the promoter, stopping transcription all of the above occur at equal rates when tryptophan levels get too high
In the tryptophan operon of E. coli, what happens when tryptophan levels get too high? tryptophan is removed from its co-repressor, causing it to detach from the operator tryptophan is removed from its co-repressor, causing it to attach to the operator tryptophan is bound to its co-repressor, causing it to detach from the operator tryptophan is bound to its co-repressor, causing it to attach to the operator all of the above occur at equal rates when tryptophan levels get too high (Option D is the answer for the question above) What effect will the above process have on the tryptophan operon? RNA polymerase will detach from the promoter, initiating transcription RNA polymerase will detach from the promoter, stopping transcription RNA polymerase will attach to the promoter, initiating transcription RNA polymerase will attach to the promoter, stopping transcription all of the above occur at equal rates when tryptophan levels get too high
. In the tryptophan operon of E. coli, what happens when tryptophan levels get too high? tryptophan is removed from its co-repressor, causing it to detach from the operator tryptophan is removed from its co-repressor, causing it to attach to the operator tryptophan is bound to its co-repressor, causing it to detach from the operator tryptophan is bound to its co-repressor, causing it to attach to the operator all of the above occur at equal rates when tryptophan levels get too high
In the tryptophan operon of E. coli, what happens when tryptophan levels get too high? tryptophan is removed from its co-repressor, causing it to detach from the operator tryptophan is removed from its co-repressor, causing it to attach to the operator tryptophan is bound to its co-repressor, causing it to detach from the operator tryptophan is bound to its co-repressor, causing it to attach to the operator all of the above occur at equal rates when tryptophan levels get too high What effect will the above process have on the tryptophan operon? RNA polymerase will detach from the promoter, initiating transcription RNA polymerase will detach from the promoter, stopping transcription RNA polymerase will attach to the promoter, initiating transcription RNA polymerase will attach to the promoter, stopping transcription all of the above occur at equal rates when tryptophan levels get too high
What is the difference between the role of the operator and the role of the promoter in an operon
when tryptophan is present, what would happen to trp operon regulation
The lac operon of E. coli controls the expression of genes that code for enzymes involved in lactose metabolism. Mutations to various regions of the lac operon have been discovered which affect both the control of gene expression and the function of the gene products. These mutations include: I - = produces a repressor protein that cannot bind to the operator region IS = produces a repressor protein that cannot be removed from the operator region OC = cannot bind a repressor protein Z - = produces a defective (non-functioning) β-galactosidase enzyme + = wild type Detail the functionality of the lac operon under the following genetic and cellular conditions. You do not need to consider the role of CAP in your analysis. Genes Repressed (yes/no) Constitutive (yes/no) IS O+ Z+ IS OC Z+
The lac operon of E. coli controls the expression of genes that code for enzymes involved in lactose metabolism. Mutations to various regions of the lac operon have been discovered which affect both the control of gene expression and the function of the gene products. These mutations include: I - = produces a repressor protein that cannot bind to the operator region IS = produces a repressor protein that cannot be removed from the operator region OC = cannot bind a repressor protein Z - = produces a defective (non-functioning) β-galactosidase enzyme + = wild type Detail the functionality of the lac operon under the following genetic and cellular conditions. You do not need to consider the role of CAP in your analysis. Genes Repressed (yes/no) Constitutive (yes/no) I+ OC Z+ I+ O+ Z+ (Lactose present) I- O+ Z+
Which of the following statements is not true of both the LacI and TrpR repressor proteins?a) an allosteric effector molecule changes the DNA-binding activity of the proteinb) transcription is blocked when the repressor protein is bound to the operatorc) the repressor protein is trans-actingd) the allosteric effector causes the repressor to release from the operatore) the operon regulated by the repressor produces the allosteric effector molecule
You are growing E. coli in a laboratory in order to study their operons. The growth media you are using contains lactose, no glucose and no tryptophan. Using your knowledge of operons and their regulation, answer the following questions Which operons would be functional under these conditions? 2. What repressors would be made? (NOTE: name the repressors using their gene names) 3.Which repressor(s) would be made in the inactive form? 4.Which repressor(s) would be made in the active form? 5.Which repressor(s) under these conditions can bind the operator sequence? 6.Which repressor(s)under these conditions cannot bind the operator sequence?
What would happen if the operator sequence of the trp operon contained a mutation that prevented the repressor protein from binding to the operator? (Explain what would happen in both the presence and absence of tryptophan)