The image above was taken using a 40x objective lens in our MoCell lab with 2.5 calibration factor. What is the size of the cell marked with red arrow? 07.5 17.5 57
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- FOV measured at 100x total magnification is 400 micrometers. What is the FOV at 40X total magnification? 2. If FOV is 5 mm and the number of cells in FOV is estimated at 35, what is the cell size in mm? In micrometers?Calculate the width of all 3 cells in micrometers. Use scale. Of a 40x objective.The culture you are working has a doubling time of 2 hours and a cell density of 3 x 106 cells per mL. For your experiment, you first dilute the culture 100 fold. Assuming that there is no lab phase and that the cells remain in exponential growth the entire time, what is the cell density (cells/mL) after 10 hours?
- The above photograph (IMAGE ATTACHED) of a cheek smear was obtained using the high-power objective of a compound microscope. What is the size of the circled cheek cell in millimeters AND nanometers?You have a culture of cells at 3.5 x 108 cells/ml and you want to prepare a fresh 5 ml culture with a starting density of 1 x 104 cells/ml. What volume of the old culture do you add to the new 5 ml culture?You have an original cell density of 4.3x10 8CF/mL. What is this number in its non-scientific notation or "regular" format?. 43,000,000CF/mL. 0.000000043CfU/mL. 430,000,000CFU/mL. 0.00000043CFU/mL. 4.3CFU/mL
- You count 4 squares of a hemacytrometer and ontain 82,65,85 cells in each squy.what is the cell density of this culture?You look through a compound microscope and count 8 cells that fit across the diameter of your field of view with the 40X lens. Your field diameter is a 4X lens and is 5 mm. Answer the following questions below: (show work for full credit): What is the field diameter at 40X? What units are you using? mm or um? ( make sure to explain your answer) What is the formula for calculating cell size?If 10 µL of cells in suspension was mixed with 20 µL of a dye called trypan blue. DF = _____. please show all calculation steps
- Using absorbance readings and a standard curve relating absorbance to cell number, you suspect that a bacterial culture contains 7.4 x 109 CFUs/ml. What dilution of this sample would give you a countable plate?Describe how you would prepare a dilution series of a 1 x 107 CFU/mL culture to the 10-8 dilution using only 4.5 mL diluents in tubes. What would be the theoretical cell count if 0.1 mL of the 2nd and 4th dilutions were each plated?What are the mechanisms of samples separation work in Thin layer Chromatography? Please shortly write at your own words. Answer should be to the point (5-6 lines maximum).