The ionization of p-nitrophenol is shown below (pKa= 7.0) a) At pH 7, what are the relative concentrations of ionized and un-ionized p-nitrophenol? b) If enough concentrated hydrochloric acid is added to a solution of p-nitrophenol to lower the pH from 7 to 5, what will happen to the relative concentrations of the ionized and un-ionized forms? c) A solution of p-nitrophenol at pH 8.3 was found to have an A400 of 0.550. What is the total concentration (in um) of p-nitrophenol (ionized plus un-ionized) in the solution? The molar extinction coefficient of p-nitrophenol is 19,500M-1cm-1 and the pKa is 7.

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The ionization of p-nitrophenol is shown below (pKa= 7.0)

a) At pH 7, what are the relative concentrations of ionized and un-ionized p-nitrophenol?

b) If enough concentrated hydrochloric acid is added to a solution of p-nitrophenol to lower the pH from 7 to 5, what will happen to the relative concentrations of the ionized and un-ionized forms?

c) A solution of p-nitrophenol at pH 8.3 was found to have an A400 of 0.550. What is the total concentration (in um) of p-nitrophenol (ionized plus un-ionized) in the solution? The molar extinction coefficient of p-nitrophenol is 19,500M-1cm-1 and the pKa is 7.

NO2
ОН
NO₂
O-
+ H+
Transcribed Image Text:NO2 ОН NO₂ O- + H+
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