The restriction endonuclease recognizes and cleaves foreign DNA sequences at specific sites, while mehtyltransferase transfers methyl groups to the same specific DNA sequence to the C-5 carbon or the N4 amino group of cytosine or to the N6 amino group of adenine. What is the purpose of the methyl-transfer reaction? How is the purpose accomplished?

The restriction endonuclease recognizes and cleaves foreign DNA sequences at specific sites, while mehtyltransferase transfers methyl groups to the same specific DNA sequence to the C-5 carbon or the N4 amino group of cytosine or to the N6 amino group of adenine. What is the purpose of the methyl-transfer reaction? How is the purpose accomplished?

Name: 5 The restriction endonuclease recognizes and cleaves foreign DNA sequ
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Description: 5 The restriction endonuclease recognizes and cleaves foreign DNA sequences at specific sites,while mehtyltransferase transfers methyl groups to the same specific DNA sequence to the C-5carbon or the N4 amino group of cytosine or to the N6 amino grou

Biology Today and Tomorrow without Physiology (MindTap Course List)

5th Edition

ISBN:9781305117396

Author:Cecie Starr, Christine Evers, Lisa Starr

Publisher:Cecie Starr, Christine Evers, Lisa Starr

Chapter10: Biotechnology

Section: Chapter Questions

Problem 1CT

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Similar questions

If restriction endonucleases are produced by bacteria within a host, why don’t these enzymes chew up the genomic DNA of their host? What is the role of DNA methyltransferase in this?
If restriction endonucleases are produced by bacteria within a host, why don’t these enzymes chew up the genomic DNA of their host? What is the role of DNA methyltransferase in this? Indicate the answer
Why would you NOT expect a restriction endonuclease to exist that would recognize the site AAGGAA?
A microbiologist discovers a new type II restriction endonuclease. When DNA is digested by this enzyme, fragments that average 1,048,500 bp in length are produced. What is the most likely number of base pairs in the recognition sequence of this enzyme?
For a restriction enzyme that recognizes the restriction site GGCC, Which of the following statements is/are true?
Restriction sites are palindromic; that is, they read the same in the5' to 3' direction on each strand of DNA. What is the advantage ofhaving restriction sites organized this way?
When circular DNA is sequenced, the nucleotide base pairs are numbered starting from a fixed position on the DNA, all the way around, usually in a clockwise manner. a DNA molecule that is 3133 base pairs long is digested with RsaI restriction enzyme recognition sites at base numbers 366, 1534, and 2207. What are the sizes of the DNA fragments that will be produced after the DNA is digested with RsaI?
What are palindromes, and how are they related to restriction endonucleases? Why are they useful for inserting one piece of DNA into another?
Which of the following sequences, when combined with itscomplement, would be clipped by a restriction endonuclease?a. ATCGATCGTAGCTA b. AAGCTTCGAA c. GAATTC d. ACCATTGGA
What feature is commonly seen in the sequences recognized by type II restriction enzymes?
Why do restriction endonucleases not hydrolyze DNA from the organism that produces it?
A 2.0kb bacterial plasmid ‘BS1030’ is digested with the restriction endonuclease Sau3A; the plasmid map is depicted in the diagram below and the Sau3A (S) restriction sites are indicated. Which of the following DNA fragments do you expect to see on an agarose gel when you run Sau3A-digested plasmid ‘BS1030’ DNA? a. 250 bp, 450 bp, 550 bp, 1.1 kb, 1.5 kb and 2.0 kb b. 2.0kb c. 250 bp, 400 bp, 450 bp, 500 bp and 550 bp d. 100 bp, 200 bp, 250 bp, 400 bp, 500 bp and 550 bp