The results of a bacterial population study done with plate dilutions were as follows: 297 colonies on the 1/1000 (10-3) plate 33 colonies on the 1/10,000 (10-4) plate What is the average titer (or original cell density)?
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The results of a bacterial population study done with plate dilutions were as follows:
297 colonies on the 1/1000 (10-3) plate
33 colonies on the 1/10,000 (10-4) plate
What is the average titer (or original cell density)?
Note: Your answer should be presented to the nearest whole number (not including decimal).
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- A 0.00001 dilution is performed on a culture of bacteria in order to perform viable plate counts. From the dilution, *0.1 mL* of solution is plated on solid media, and 223 colonies of bacteria grow on the petri dish. Assuming each colony came from a single bacterium, how many bacteria are in a single mL of the original culture? Express your answer to two decimal places using exponential notation. Since only 0.1 mL is put on the plate, this counts as an extra dilution!!! Any time less than 1 mL is transfered, a dilution is being performed. Any time more than 1 mL is transfered, a concentration is being performed.With reference to this data what is the number of samples (n)?What are the reasons for a biased sample
- What is the difference between a random sample and a composite sample? When would each be used?you know that 1.1OU=1um. you have an organism that measures at 12 O.U at 10x. How long is your organism? assume O.U were calibrated at 1000x.A 10-2 is performed on a culture of bacteria in order to perform viable plate counts. From the dilution, *0.1 mL* of solution is plated on solid media, and 61 colonies of bacteria grow on the plate. Assuming each colony came from a single bacterium, how many bacteria are in a single mL of the original culture?
- Do you think that each sample occupies space? Explain whyThe table here is based on the published results of the study discussed in the chapter. It shows the number of bacterial phylotypes (kinds of bacteria) that were found on a particular percentage of people. a. How many bacterial phylotypes were found on only 10 percent or fewer of the people sampled? How many were found on more than 90 percent of people? b. How many phylotypes were found on more than half of the people sampled? c. Describe in one or two sentences the frequency of phylotypes found in the study. Were most phylotypes rare or common?What is an example of a measurement error statment for doing an experiment dealing with counting Colony forming units?
- What if all the data above was the same EXCEPT with the change of G & X? Their new data is below. Would base X still be T? G & X [G] = 0.0447315M [X] = 0.0447315M [GX] = 0.0026845M 1.3416During the Lederberg and Tatum experiment, theresearchers compared the growth of mutant strains under twoscenarios: mixed strains or unmixed strains. When the unmixedstrains were plated on the experimental growth medium, whywere no colonies observed to grow? When the mixed strainswere plated on the experimental growth medium, 10 colonieswere observed. What was the significance of these colonies?Why is there a blue scale?