The separation of molecules within a gel is determined by the relative size of the pores formed within the gel. Thus, the gel percentage depends on the size of your protein of interest. Protein size (kDa) Gel percentage (%) 4-40 20 12-45 15 10-70 12.5 15-100 10 25-200 8

The separation of molecules within a gel is determined by the relative size of the pores formed within the gel. Thus, the gel percentage depends on the size of your protein of interest. Protein size (kDa) Gel percentage (%) 4-40 20 12-45 15 10-70 12.5 15-100 10 25-200 8

Chemistry: The Molecular Science

5th Edition

ISBN:9781285199047

Author:John W. Moore, Conrad L. Stanitski

Publisher:John W. Moore, Conrad L. Stanitski

Chapter13: The Chemistry Of Solutes And Solutions

Section: Chapter Questions

Problem 83QRT

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Concept explainers

Mass Spectrometry

This process is an instrumental method in which a sample is changed to fast moving positive ions by electron bombardment. The charged particles vary due to their different mass to charge ratio and are estimated with the help of this difference. It is also called mass spectroscopy.

Question

For leptin, what gel percentage are you going to prepare?

The separation of molecules within a gel is determined by the relative size of the pores
formed within the gel. Thus, the gel percentage depends on the size of your protein of
interest.
Protein size (kDa)
Gel percentage (%)
4-40
20
12-45
15
10-70
12.5
15-100
10
25-200
8

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