The thyroid-stimulating hormone thyrotropin has the following sequence: Met-Asp-Tyr-Tyr-Arg Lys Tyr-Ala-Ala-Val Calculate the molecular weight of thyrotropin, using data in the table below. TABLE 5.1 Properties of the common amino acids found in proteins Name Alanine Arginine Asparagine Aspartic acid Cysteine Glutamine Glutamic acid Glycine Histidine Isoleucine Leucine Lysine Methionine Phenylalanine Proline Serine Threonine Tryptophan Tyrosine Valine Abbreviations 1-3- pk of letter codes A, Ala R, Arg N, Asn D, Asp C, Cys Q, Gln E, Glu a a 9.7 8.7 9.0 8.8 5.0 4.2 5.9 1.3 9.8 10.8 9.1 3.7 9.7 6.6 9.6 7.9 9.2 2.4 9.7 5.5 9.6 8.9 9.0 5.5 9.2 2.0 9.1 4.0 10.6 97.12 4.7 9.2 87.08 5.8 10.4 101.11 5.6 2.4 9.4 186.21 1.5 2.2 9.1 163.18 3.5 2.3 9.6 99.14 7.2 "Approximate values found for side chains on the free amino acids. W.P. Jencks and J. Regenstein (1976) lonization constants of acids and bases in Handbook of Biochemistry and Molecular Biology, 3rd ed., G. Fasman (ed.), CRC Press, Boca Raton, FL. G, Gly H, His I, Ise L, Leu K, Lys M, Met F, Phe P, Pro S, Ser T, Thr W, Trp Y, Tyr V, Val PK, of COOH Group a-NH3+ Group PK of lonizing Residue Mass Occurance in Side Chain (daltons) Proteins (mol %) 2.3 2.2 2.0 2.1 1.8 2.2 2.2 2.3 1.8 2.4 2.4 2.2 2.3 1.8 2.0 2.2 2.6 a 12.5 3.9 8.3 4.2 6.0 10.0 10.1 71.08 156.20 114.11 115.09 103.14 128.14 129.12 57.06 137.15 113.17 113.17 128.18 131.21 147.18 To obtain the mass of the amino acids itself, add the mass of a molecule of water, 18.02 daltons. The values given are for neutral side chains; slightly different values will apply at pH values where protons have been gained or lost from the side chains. "Average for a large number of proteins. Individual proteins can show large deviations from these values. Data from J. M. Otaki, M. Tsutsumi, T. Gotoh, and H. Yamamoto, Secondary structure characterization based on amino acid composition and availability in proteins. (2010) Journal of Chemical Information and Modeling 50:690 700 2010 American Chemical Society. Express your answer to two decimal places and include the appropriate units.

The thyroid-stimulating hormone thyrotropin has the following sequence: Met-Asp-Tyr-Tyr-Arg Lys Tyr-Ala-Ala-Val Calculate the molecular weight of thyrotropin, using data in the table below. TABLE 5.1 Properties of the common amino acids found in proteins Name Alanine Arginine Asparagine Aspartic acid Cysteine Glutamine Glutamic acid Glycine Histidine Isoleucine Leucine Lysine Methionine Phenylalanine Proline Serine Threonine Tryptophan Tyrosine Valine Abbreviations 1-3- pk of letter codes A, Ala R, Arg N, Asn D, Asp C, Cys Q, Gln E, Glu a a 9.7 8.7 9.0 8.8 5.0 4.2 5.9 1.3 9.8 10.8 9.1 3.7 9.7 6.6 9.6 7.9 9.2 2.4 9.7 5.5 9.6 8.9 9.0 5.5 9.2 2.0 9.1 4.0 10.6 97.12 4.7 9.2 87.08 5.8 10.4 101.11 5.6 2.4 9.4 186.21 1.5 2.2 9.1 163.18 3.5 2.3 9.6 99.14 7.2 "Approximate values found for side chains on the free amino acids. W.P. Jencks and J. Regenstein (1976) lonization constants of acids and bases in Handbook of Biochemistry and Molecular Biology, 3rd ed., G. Fasman (ed.), CRC Press, Boca Raton, FL. G, Gly H, His I, Ise L, Leu K, Lys M, Met F, Phe P, Pro S, Ser T, Thr W, Trp Y, Tyr V, Val PK, of COOH Group a-NH3+ Group PK of lonizing Residue Mass Occurance in Side Chain (daltons) Proteins (mol %) 2.3 2.2 2.0 2.1 1.8 2.2 2.2 2.3 1.8 2.4 2.4 2.2 2.3 1.8 2.0 2.2 2.6 a 12.5 3.9 8.3 4.2 6.0 10.0 10.1 71.08 156.20 114.11 115.09 103.14 128.14 129.12 57.06 137.15 113.17 113.17 128.18 131.21 147.18 To obtain the mass of the amino acids itself, add the mass of a molecule of water, 18.02 daltons. The values given are for neutral side chains; slightly different values will apply at pH values where protons have been gained or lost from the side chains. "Average for a large number of proteins. Individual proteins can show large deviations from these values. Data from J. M. Otaki, M. Tsutsumi, T. Gotoh, and H. Yamamoto, Secondary structure characterization based on amino acid composition and availability in proteins. (2010) Journal of Chemical Information and Modeling 50:690 700 2010 American Chemical Society. Express your answer to two decimal places and include the appropriate units.

Biochemistry

6th Edition

ISBN:9781305577206

Author:Reginald H. Garrett, Charles M. Grisham

Publisher:Reginald H. Garrett, Charles M. Grisham

Chapter25: Nitrogen Acquisition And Amino Acid Metabolism

Section: Chapter Questions

Problem 13P

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