The titer of Lauren's phage is 3.7 X 108 pfu/mL. She diluted her phage lysate 1:10 from 10° to 10-7 and use 10μL of lysate for each infection as she followed the procedure for a titer assay.
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- A probe is a full section of DNA or RNA that has been labeled with a tracer. True FalseBacteriophage A average = 136 plaques Bacteriophage B average = 38 plaques Bacteriophage C average = 27 plaques Please help find the PFU/ml and answer the question on the best bacteriophage. ThanksOrder: streptomycin 500 mg IM daily for 8 days. If you have vials of this antibiotic that are labeled as 1 g/2.5 mL, how many milliliters would you administer?
- Serial dilutions are made in order to determine the level of the antibody in the sample. True or False ?The following pictures show the results of a Disk Diffusion Assay for different types of bacteria. For each bacteria, what antibiotic would you recommend be used on the patient? Explain your choice.You are counting plaques on your plaque assay plates made from serial dilutions of your high titer lysate. Your 10-5 plate has 615 plaques although some are butting up against each other so it is difficult to get an accurate count. Your 10-6 plate has 42 plaques, and your 10-7 plate has only 1 plaque. Which plate would probably yield the most accurate titer calculation of your phage and why is it more trustworthy than the others?
- What is the immunoassay analyzer MachineA 10-2 is performed on a culture of bacteria in order to perform viable plate counts. From the dilution, *0.1 mL* of solution is plated on solid media, and 61 colonies of bacteria grow on the plate. Assuming each colony came from a single bacterium, how many bacteria are in a single mL of the original culture?Please choose the correct answer. Initial DNA nucleotides are added by reverse transcriptase from the primer binding site to the a. uracil rich 5’ end. b. uracil rich 3’ end. c. polypurine tract. d. ssDNA.
- Counterstaining with Hematoxylin and Eosin is an important step in which of the following techniques? ELISA Gel Electrophoresis IHC Western blottingWhat does BCR ABL positive mean?Prepare a dilution of phage from 1.5 x 1010 PFU/mL to 1.00 x 102 PFU/mL. Draw a dilution scheme to achieve this serial dilution considering the following: The volumes of each dilution should be 1.0mL. It is not advisable to perform any dilutions > 1/100. The first dilution in any titre determination of stock phage should use 10µl or less of stock.