There is a rare microalga that has a promising future as a producer of a new type of biofuel. After research in the lab, it is found that it is having a difficult time propagating outside of its native environment, and it is identified that this is an issue with its respiration pathway. Overall ATP production is around half of expected, and you find that glycolysis isn’t producing any net ATP molecules and only one molecule of NADH. Question: Assuming that the respiration pathway is similar to other prokaryotes, where would you expect to find a nonfunctional mutation in this pathway? What is the reason for this? What could a growth strategy be that can better support this rare bacterium until you can come up with a long-term solution?

Biology Today and Tomorrow without Physiology (MindTap Course List)
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ISBN:9781305117396
Author:Cecie Starr, Christine Evers, Lisa Starr
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Chapter13: Early Life Forms And The Viruses
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There is a rare microalga that has a promising future as a producer of a new
type of biofuel. After research in the lab, it is found that it is having
a difficult time propagating outside of its native environment, and it is identified that this
is an issue with its respiration pathway.

Overall ATP production is around half of expected,
and you find that glycolysis isn’t producing any net ATP molecules and only
one molecule of NADH.

Question: Assuming that the respiration pathway is similar to other prokaryotes, where
would you expect to find a nonfunctional mutation in this pathway? What is the reason for this? What could a growth strategy be that can better support this
rare bacterium until you can come up with a long-term solution?

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The seocond part of this question is: how could you find a way to improve the metabolic efficiency of this microalga if it is going to be feasible for the biofuel industry? Using the target gene you identified above, explain a CRISPR-based strategy you could use to restore its function.   

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