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- The cells from a person’s malignant tumor were subjected to insitu hybridization using a probe that recognizes a unique sequenceon chromosome 14. The probe was detected only once in each ofthe cells. Explain this result, and speculate on its significance withregard to the malignant characteristics of these cells.Give at least three examples of types of mutationsthat would disrupt the process of mitotic chromosomesegregationWith regard to the analysis of chromosome structure, explain theexperimental advantage that polytene chromosomes offer. Discusswhy changes in chromosome structure are more easily detected inpolytene chromosomes than in ordinary chromosomes.
- Describe when X-chromosome inactivation occurs and how thisleads to phenotypic results at the organism level. In your answer,you should explain why XCI causes results such as variegated coatpatterns in mammals. Why do two different calico cats have theirpatches of orange and black fur in different places? Explainwhether or not a variegated coat pattern due to XCI could occur inmarsupials.In an electrophoretic gel across which is applied a powerful electrical alternating pulsed field, the DNA of thehaploid fungus Neurospora crassa (n = 7) moves slowlybut eventually forms seven bands, which represent DNAfractions that are of different sizes and hence havemoved at different speeds. These bands are presumed tobe the seven chromosomes. How would you show whichband corresponds to which chromosome?In which of the following is genetic material moved betweennonhomologous chromosomes?a. insertion d. translocationb. nondisjunction e. inversionc. deletion
- For each of the terms in the left column, choose thebest matching phrase in the right column.a. reciprocal translocation 1. lacking one or morechromosomes or having oneor more extra chromosomesb. gynandromorph 2. movement of short DNAelementsc. pericentric 3. having more than two completesets of chromosomesd. paracentric 4. exact exchange of parts of twononhomologous chromosomese. euploids 5. excluding the centromeref. polyploidy 6. including the centromereg. transposition 7. having complete sets ofchromosomesh. aneuploids 8. mosaic combination of maleand female tissueA female fruit fly has one normal X chromosome and one X chromosomewith a deletion. The deletion occurred in the middle ofthe X chromosome and removed about 10% of the entire length ofthe X chromosome. Suppose you stained and observed the chromosomesin salivary gland cells of this female fruit fly. Draw thepolytene arm of the X chromosome. Explain your drawing.A chromosome territory is a regiona. along a chromosome where many genes are clustered.b. along a chromosome where the nucleosomes are closetogether.c. in a cell nucleus where a single chromosome is located.d. in a cell nucleus where multiple chromosomes are located
- Why is a chromosome with two centromeres (adicentric chromosome) unstable? Would a backup cen-tromere not be a good thing for a chromosome, giving ittwo chances to form a kinetochore and attach to microtu-bules during mitosis? Would that not help to ensure thatthe chromosome did not get left behind at mitosis?A male Drosophila from a wild-type stock is discovered to haveonly seven chromosomes, whereas normally 2n = 8. Closeexamination reveals that one member of chromosome IV (thesmallest chromosome) is attached to (translocated to) the distalend of chromosome II and is missing its centromere, thusaccounting for the reduction in chromosome number. (a) Diagram all members of chromosomes II and IV duringsynapsis in meiosis I.A colleague e-mails you saying that she has identified an interesting chromosome variation at 21q13. In discussing this discovery with a friend who is not a cytogeneticist, explain how you would describe this location, defining each term in the chromosome address 21q13.