Tube 1: Add 1 mL 5% Trypsin Tube 2: Add 1 mL 5% Trypsin and 2 mL of 0.5% Na2CO3 Solution Tube 3: Add 2 mL 0.5% Na2CO3 Solution 2. Place all test tubes in hot water bath maintained at 38-40°C for one hour. 3. Filter the contents of each test tube and use the filtrate for biuret test.  Question: What are the possible color change that can be observed in the experiment? In which of the test tubes did hydrolysis of protein take place?

Curren'S Math For Meds: Dosages & Sol
11th Edition
ISBN:9781305143531
Author:CURREN
Publisher:CURREN
Chapter21: Heparin Infusion Calculations
Section: Chapter Questions
Problem 3.8P
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Experiment: Action of Pancreatic Proteases and Trypsin on Protein

Procedure:

B. Action of Pancreatic Proteases and Trypsin on Protein
1. Prepare 3 test tubes containing 2 mL egg albumin solution.
Tube 1: Add 1 mL 5% Trypsin
Tube 2: Add 1 mL 5% Trypsin and 2 mL of 0.5% Na2CO3 Solution
Tube 3: Add 2 mL 0.5% Na2CO3 Solution
2. Place all test tubes in hot water bath maintained at 38-40°C for one hour.
3. Filter the contents of each test tube and use the filtrate for biuret test. 

Question: What are the possible color change that can be observed in the experiment? In which of the test tubes did hydrolysis of protein take place?

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