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Uninoculated "controls" for each medium should score 0 (zero) in their respective tubes. Explain the importance of this affirmation 9, x10
in microbiology , we try to culture microorganims . for this purpose we use various culture media . the culture media can be either in a plate or a tube in which a agar solution with suitable agents for the growth are provided .
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- 15. Colony formation can be observed in liquid media. a) True b) FalseTrue / False 1. Sterilization can penetrate most soil, so there is no need to clean a surgical instrument prior to sterilization. 2. One of the greatest challenges in infection control of reprocessed medical devices is a lack of consistent understanding of what the definition of "clean" is.You counted 4, 6, 12, 3 cells in each of the 4.outed squares of a hemacytomeyer. What are the cells per milliliter in that culture? If you resuspended your cell pellet 2.5 mL, what is the total cell count? How many uL do you need to add to a new culture if you want 4250 cells?
- a. What is the total dilution of Tube #4? Express the answer in exponential format. b. You plated 1 mL of the Tube #4, After incubating, you counted 500 colonies on the plate. What is the concentration of Tube #0? include units. c. How could you change the experiment in part B to get a plate in the countable range? Be specific about any dilution factors and/or plated volumes you would change.Please note that these counts have all been done with Trypan Blue. Therefore, all cultures have been diluted two-fold (equal volume of cell suspension + equal volume of Trypan Blue). Take this into account when determining cell number. Any dilution listed in table is an additional dilution. Live and dead cells listed below represent the total number of cells in 5 large counting squares. *1 x 105 cells/ml cells were seeded into each well of the 24 well plate(s) on day 0 to begin the growth study. Plot this value at the zero time point on your graph.1These numbers represent the total number of cells counted in 5 large squares from three separate counts.How can I explain the results of my findings in lamen terms? 1 - Name of Test Type of Medium 2 - Color Before Incubation 3-Color After Incubation? 4 – Results Positive/ Negative? 5 - Reagents Added Lactose Fermentation test Phenol Red Lactose (PRL) Red Color: Yellow Gas Bubble: Yes Positive Phenol Red to detect color change (added) Glucose Fermentation Test Phenol Red Glucose (PRG) Red Color: Yellow Gas Bubble: Yes Positive Phenol Red to detect color change (added) Citrate Utilization Simmons Citrate Green Color: Blue Positive Bromthymol Blue (added) Indole Test Media = SIM Clear Indole: Color: Clear Negative For Indole, we add Kovac’s Hydrogen Sulfide Test Media = SIM clear Hydrogen Sulfide: Color: Clear Negative Iron already added Methyl Red Test (MR test) Media = MRVP Broth Clear Color: Yellow Negative We add Methyl Red Reagent Voges-Proskauer Test (VP test) Media = MRVP Broth Clear…
- The solution containing bacterial spores is heated in an autoclave. When the autoclave has reached a constant temperature of 121 °C, there are still 106 spores/ml. The specific death constant for bacterial spores at 121 ° C is 11.62 min-1. How long should the heating be continued at a constant temperature so that there are 1 spores/ml left? Enter the answer in minutes to three decimal places.The predominance of squamous epithelial cells with few WBCs in a sputum sample would be adequate for diagnosis and culture. true falsevolume of the quasi-steady-state culture was V0= 500 L, and the nutrient solution containing glucose was added at a constant flow rate of F = 50 L/h.Data: X0 (at the beginning of feeding) = 20 g/L, S0 = 300 g/L, max = 0.2 h-1, KS = 0.5 g/L and Y x/s= 0.3 g/g a) Determine the volume of the culture at t = 10hb) Determine the concentration of glucose at t = 10 hc) determine the concentration and total mass of cells at t = 10 hd) If product is associated with growth with α = 1.5 and P0 = 0.1 g/L, determine the concentration of product at t = 10h. (answer:P = 67,55 g/L)
- What is the clinical significance of ESR determination? Differentiate Wintrobe from Westergreen regarding accuracy of the test in a tabulated order. NOTE: Kindly asnwer all the questions. Thank you!A 76-year-old woman presents today with complaints of nasal drainage, clearing of throat, and occasional nasal congestion, especially on waking in the morning. She has recently moved into an independent living center after living in her home for 40 years. She states that, although she has had these symptoms before, generally the symptoms appeared in the spring, and she associated the nasal drainage with pollination. Because it is winter, she could not identify the trigger of her symptoms what additional specific laboratory tests wil you consider ordering?N. benthamiana will be infiltrated with a solution containing OD600=0.3 of each experimental Agro containing construct and OD600=0.1 of p19. Calculate the volume of cultures (V construct; V p19) needed according to the formulas: V construct = V final × 0.3/OD600; V p19 = V final × 0.1/OD600. One mL of infiltrate is often enough to complete a small experiment. How to plan your final volume accordingly?