You counted 4, 6, 12, 3 cells in each of the 4.outed squares of a hemacytomeyer. What are the cells per milliliter in that culture? If you resuspended your cell pellet 2.5 mL, what is the total cell count? How many uL do you need to add to a new culture if you want 4250 cells?
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You counted 4, 6, 12, 3 cells in each of the 4.outed squares of a hemacytomeyer. What are the cells per milliliter in that culture? If you resuspended your cell pellet 2.5 mL, what is the total cell count? How many uL do you need to add to a new culture if you want 4250 cells?
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- With a plate count average of 297 and a final dilution on the plate of 1:1,000 ; what is the viable number of cells in original culture (CFU/ml)?Steven is a lab research assistant. He mixed 25 µL of cells with 50 µL of cell culture media in tube-A, then transferred 10 µL to the hemocytometer and started counting cells, then realized the cells were too crowded to count accurately, so Steven took an aliquot of 20 µL cells of cells from tube-A and mixed with 180 µL of cell culture media, transferred 10 µL onto the hemocytometer, and countered 500 cells from the squares on the four corners of the hemacytometer. What is the # of cells per mL? Please show all calculation steps.If there is a sample having 3.5x10^5 CFU/ml, and this cell count came from a sample of the original culture which had been diluted 1x10^-4 times, what is the number of CFU/ml of the original culture?
- "If you count 70 colonies from a urine culture obtained using 0.001ml calibrated loop, how many colony forming units (CFU) will be reported?" 70 700 7000 70000 700000If the 1-mL sample had been added to 99 mL of diluent to make the first dilution (instead of 9 mL), how many cells would be in that particular dilution?In this experiment, a culture was serially diluted to the concentrations below. Each plate was plated with 0.25mL of the dilution. Using the most diluted plate, what is the correct concentration of the original culture? A) 2.0 X 10^-3 cells/mL B) 2.0 X 10^5 cells/mL C) 4.0 X 10^5 cells/mL D) 5.0 X 10^4 cells/mL
- one lm of milk was added to 9 ml of sterile saline. 0.1ml of the diluted sample was put into a petri dish with melted agar. How many cfu/ml are in the milk if 200 colonies were counted?Plating 0.1 mL of a sample diluted by a factor of 10^(-3) produced 43 colonies. What was the original concentration (OCD) in the undiluted sample?In a plate count, 1 mL of culture is spread on a plate and incubated for 24 hours. 250 colonies are counted. Answer the question below. there were _____cells in the 1 mL of culture that was spread on the plate. If the culture was diluted 1:100 prior to plating, how many cells were there per mL in the original culture? _________
- If you plate 200 ul of a 1:100,000 dilution and get 123 colonies, what is the number of CFU/mL in the original sample?A 2x10-6 dilution of your bacterial culture yielded 10 colonies. What was the cell density (incells/ml) of your bacterial culture?You count 4 squares of a hemacytrometer and ontain 82,65,85 cells in each squy.what is the cell density of this culture?